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首页> 外文期刊>Modern Chemistry >The Effect of pH on the Accumulation of Astaxanthin in Haematococcus Pluvialis in the Induction Stage
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The Effect of pH on the Accumulation of Astaxanthin in Haematococcus Pluvialis in the Induction Stage

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摘要

In order to explore the influence of Haematococcus pluvialis on the accumulation of astaxanthin in the medium pH environment during the induction phase, two algae species bank algae liquids were selected (the algae species bank established by Shanghai Guangyu Biotechnology Co, Freshwater Algae Species Bank of Chinese Academy of Sciences) through the method of comparative experiment to explore the medium composition (modified BG11) in the same ratio, the light intensity is 240µmol·m-2·s-1, the temperature is 15~25°C, through citric acid and hydroxide Sodium solution is used to adjust the algae environment with a small gradient of pH 6.5, 7, and 7.5 respectively. Regularly take the algae fluid to detect and record the absorbance value and the average cell radius of Haematococcus pluvialis cells, observe the color of the algae fluid, the induction effect, and record the red blood cell data generated by Haematococcus pluvialis, and then use the least squares curve to simulate Collect data to show the accumulation of astaxanthin and subsequent experimental verification. It is concluded that the astaxanthin accumulation effect of the freshwater algae species bank with a pH of 6.5 under this comparative environment is better than that of the other two pH environments, and the freshwater algae species bank algae fluid has a better astaxanthin accumulation effect in this environment Guangyu's conclusion of the species library algae liquid.

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