...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Proteins and Proteomics >Mass spectrometric profiling of tryptic digests of trifluoroethanol extracts from core needle biopsies of breast cancer tissues is a viable sample screening tool for biomarker discovery
【24h】

Mass spectrometric profiling of tryptic digests of trifluoroethanol extracts from core needle biopsies of breast cancer tissues is a viable sample screening tool for biomarker discovery

机译：

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Abstract Application of classifying limited sampling biopsies by matrix-assisted laser desorption/ionization time-of-flight (MALDI-ToF) based mass spectral profiling prior to liquid chromatography–tandem mass spectrometry (LC–MS/MS) analysis was studied. The trifluoroethanol (TFE) isolates from core needle biopsies from stage II (nonmetastatic) and stage IV (metastatic) breast tumors were subjected to trypsin digestion and the peptides were profiled on MALDI-ToF. The p value for TFE-based isolation method induced, intra- and inter-day variability (range 0.69–1.00) indicated reproducible protein isolation. MALDI-ToF mass spectrometry profiling of peptides showed five discriminant peaks with fold change > 2 or < 0.5 and p value < 0.05, between stage II and stage IV primary tumors. The supervised neural network (SNN) model showed the best recognition capability and cross validation with 91.67 and 69.69, respectively. The models were validated using an independent sample set and found that genetic algorithm (GA) had the highest sensitivity and SNN had the highest specificity. LC–MS/MS was performed on the classified sample sets. One of the distinguishing peaks in the MALDI analysis (m/z 1570.99) coincided with the peptide derived from vimentin, a known metastatic marker. Using western blotting, along with mimecan, the two markers showed differential levels between the groups validating the classification. MALDI-ToF/ToF-based peptide profiling of TFE lysates from core needle breast biopsy may serve as a screening tool for further deep proteomic analysis.

著录项

相似文献

  • 外文文献
  • 中文文献
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号