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The conjugation of amyloid beta protein on the gold colloidal nanoparticles' surfaces

机译：淀粉样β蛋白在金胶体纳米颗粒表面上的缀合

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摘要

The conjugation of various sequences of amyloid beta protein solution (A beta); A beta_(1-11), A beta_(12-28), A beta_(31-35), A beta_(1-40), and A beta_(1-42) with gold colloidal suspension of 20 nm size was examined. Absorption spectroscopy was utilized to identify changes in the optical properties of gold colloid for pHs, ranging from pH 2 to pH 10. Colour changes were seen for all tested proteins in this study at a higher pH than where bare gold colloid exhibits its colour change at pH = 3.09 +- 0.02. All tested A beta sequences except for A beta_(1-42) exhibited colour changes around pI values of A beta_(1-40), about pH 5.2. The A beta_(1-42) exhibited precipitants in all pH lower than pH 7 and showed the colour change around pH 3.96 +- 0.05. The colour change observed at a pH lower than 5 is attributed to the unfolded A beta3 monomer units around the gold colloidal surface. Interestingly, only A beta_(1-40)-coated gold colloidal nanoparticles exhibited a reversible colour change as the pH was externally altered between pH 4 and 10. This reversibility is an important implication of the observation of a reversible step reported for the fibrillogenesis. It was interpreted that the reversible process takes place when hydrophilic A beta possesses a three-dimensional network containing both beta-sheet and alpha-helices.
机译：淀粉样蛋白溶液（A beta）的各种序列的缀合;检查了具有20 nm大小的金胶体悬浮液的beta_（1-11），A beta_（12-28），A beta_（31-35），A beta_（1-40）和A beta_（1-42） 。利用吸收光谱法确定了金胶体在pH范围从2到pH 10的光学特性中的变化。在高于pH值的裸胶体表现出色变的情况下，本研究中所有测试蛋白质的颜色均发生了变化。 pH ＝ 3.09±0.02。除A beta_（1-42）外，所有测试的A beta序列均在约pH 5.2的A beta_（1-40）的pI值附近出现颜色变化。在所有低于pH 7的pH中，A beta_（1-42）都会显示出沉淀剂，并且在pH 3.96±0.05左右时会显示颜色变化。在低于5的pH值下观察到的颜色变化归因于金胶体表面周围未折叠的A beta3单体单元。有趣的是，当pH从外部在pH 4到10之间改变时，只有A beta_（1-40）包覆的金胶体纳米颗粒表现出可逆的颜色变化。这种可逆性是观察到的针对原纤维形成的可逆步骤的重要观察结果。据解释，当亲水性Aβ具有一个包含β-sheet和α-螺旋的三维网络时，可逆过程就会发生。

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