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Biofunctionalization of CeF_3:Tb~(3+)nanoparticles

机译:CeF_3:Tb〜(3+)纳米粒子的生物功能化

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CeF_3 :Tb~(3+) nanoparticles (short pillar-like morphology with an average length and width of 11 and 5 nm, respectively) were successfully prepared by a polyol process using diethyleneglycol (DEG) as solvent. After being functionalized with a SiO_2-NH_2 layer, these CeF_3 :Tb~(3+) nanoparticles can be conjugated with biotin molecules (activated by thionyl chloride) and further with avidin. The as-formed CeF_3:Tb~(3+) nanoparticles, CeF_3:Tb~(3+) nanoparticles functionalized with amino groups, biotin conjugated amino-functionalized CeF_3:Tb~(3+) nanoparticles and biotinylated CeF_3:Tb~(3+) nanoparticles bonded with avidin were characterized by x-ray diffraction (XRD), transmission electron microscopy (TEM), Fourier transform infrared (FT-IR), UV/vis absorption spectra and luminescence spectra, respectively. The biofunctionalization of the CeF_3 :Tb~(3+) nanoparticles has less effect on their luminescence properties, i.e. they still show strong green emission (from Tb~(3+), with ~5D_4 - ~7F_5 at 543 nm as the most prominent group), indicative of the great potential for these CeF_3:Tb~(3+) nanoparticles to be used as biological fluorescence probes.
机译:以二甘醇(DEG)为溶剂,通过多元醇法成功制备了CeF_3:Tb〜(3+)纳米粒子(分别为短柱状形态,平均长度和宽度分别为11和5 nm)。这些CeF_3:Tb〜(3+)纳米粒子在被SiO_2-NH_2层官能化后,可以与生物素分子(被亚硫酰氯激活),以及与抗生物素蛋白结合。形成的CeF_3:Tb〜(3+)纳米粒子,氨基官能化的CeF_3:Tb〜(3+)纳米粒子,生物素共轭的氨基官能化的CeF_3:Tb〜(3+)纳米粒子和生物素化的CeF_3:Tb〜(3 +)与抗生物素蛋白结合的纳米颗粒分别通过X射线衍射(XRD),透射电子显微镜(TEM),傅立叶变换红外(FT-IR),UV / vis吸收光谱和发光光谱进行表征。 CeF_3:Tb〜(3+)纳米粒子的生物功能化对其发光特性的影响较小,即它们仍显示出强烈的绿色发射(来自Tb〜(3+),在543 nm处的〜5D_4-〜7F_5最突出。组),表明这些CeF_3:Tb〜(3+)纳米粒子可用作生物荧光探针的巨大潜力。

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