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Carbon nanotube-based labels for highly sensitive colorimetric and aggregation-based visual detection of nucleic acids

机译：基于碳纳米管的标记物，用于核酸的高度灵敏的比色和基于聚集的视觉检测

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摘要

A novel carbon nanotube (CNT) derived label capable of dramatic signal amplification of nucleic acid detection and direct visual detection of target hybridization has been developed. Highly sensitive colorimetric detection of human acute lymphocytic leukemia ( ALL) related oncogene sequences amplified by the novel CNT-based label was demonstrated. Atomic force microscope (AFM) images confirmed that a monolayer of horseradish peroxidase and detection probe molecules was immobilized along the carboxylated CNT carrier. The resulting CNT labels significantly enhanced the nucleic acid assay sensitivity by at least 1000 times compared to that of conventional labels used in enzyme-linked oligosorbent assay (ELOSA). An excellent detection limit of 1 x 10(-12) M ( 60 x 10(-18) mol in 60 mu l) and a four-order wide dynamic range of target concentration were achieved. Hybridizations using these labels were coupled to a concentration-dependent formation of visible dark aggregates. Targets can thus be detected simply with visual inspection, eliminating the need for expensive and sophisticated detection systems. The approach holds promise for ultrasensitive and low cost visual inspection and colorimetric nucleic acid detection in point-of-care and early disease diagnostic application.
机译：已开发出一种新颖的碳纳米管（CNT）衍生的标记，该标记能够显着地扩增核酸检测信号并直接目测检测目标杂交。新型的基于CNT的标记扩增了人类急性淋巴细胞白血病（ALL）相关癌基因序列的高灵敏度比色检测方法。原子力显微镜（AFM）图像证实，辣根过氧化物酶和检测探针分子的单层分子被固定在羧基化CNT载体上。与用于酶联寡吸附测定（ELOSA）的常规标记相比，所得的CNT标记显着提高了核酸测定灵敏度至少1000倍。达到了1 x 10（-12）M的极佳检测限（60μl中60 x 10（-18）mol）和目标浓度的四阶宽动态范围。使用这些标记的杂交与可见暗聚集体的浓度依赖性形成偶联。因此，可以通过目测简单地检测目标，从而无需昂贵且复杂的检测系统。该方法有望在现场护理和早期疾病诊断应用中进行超灵敏，低成本的目视检查和比色核酸检测。

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