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Functionalized multi-walled carbon nanotubes as affinity ligands

机译：功能化的多壁碳纳米管作为亲和配体

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摘要

Functionalization of carbon nanotubes is very challenging for their applications. The paper here describes a new method to functionalize multi-walled carbon nanotubes (MWCNTs) as specific affinity adsorbents. MWCNTs were acid purified and pretreated with (3-aminopropyl)-triefhoxysilane (APTES) in order to introduce abundant amino groups on the surface of MWCNTs. After the conversion of amino groups to carboxyl groups by succinic acid anhydride, MWCNTs were attached to protein A or aminodextran using l-ethyl-3, 3' (dimethylamion)-propylcarbodiimide as a biofunctional crosslinker. The incorporation of aminodextran as a spacer arm noticeably increased the binding capacity of the APTES-modified MWCNTs for protein A. The application of affinity MWCNTs for purification of immunoglobulin G was then evaluated. The affinity of MWCNTs with AMD spacer exhibited a high adsorption capacity of approx361 mu g IgG/mg MWCNT (wet basis). About 75 percent of bound IgG was eluted from affinity MWCNTs (ANT-I and ANT-II) and ELIS A confirmed that the biological activity of IgG was well preserved during the course of affinity separation. The functionalized MWCNTs could be potentially used in affinity chromatography.
机译：碳纳米管的功能化对于其应用而言非常具有挑战性。本文在此介绍了一种将多壁碳纳米管（MWCNT）功能化为特定亲和吸附剂的新方法。 MWCNT经过酸纯化，并用（3-氨基丙基）-三乙氧基硅烷（APTES）进行预处理，以便在CNT的表面引入大量的氨基。在通过琥珀酸酐将氨基转化为羧基后，使用1-乙基-3，3'（二甲基酰胺）-丙基碳二亚胺作为生物功能交联剂，将MWCNTs附着于蛋白A或氨基葡聚糖。氨基葡聚糖作为间隔臂的结合显着提高了APTES修饰的MWCNT与蛋白A的结合能力。然后评估了亲和MWCNT在纯化免疫球蛋白G中的应用。 MWCNT与AMD间隔基的亲和力表现出约361μgIgG / mg MWCNT（湿基）的高吸附能力。从亲和MWCNT（ANT-1和ANT-1）洗脱出约75％的结合IgG，ELIS A证实在亲和力分离过程中，IgG的生物学活性得到了很好的保存。功能化的MWCNT可以潜在地用于亲和色谱中。

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