• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Nanotechnology >Enhanced DNA detection based on the amplification of gold nanoparticles using quartz crystal microbalance
【24h】

Enhanced DNA detection based on the amplification of gold nanoparticles using quartz crystal microbalance

机译：基于使用石英晶体微量天平扩增金纳米颗粒的增强型DNA检测

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Gold nanoparticles were used as mass amplifiers to improve the frequency signal of QCM detection of DNA. Indirect labelling and direct labelling of DNA probes with gold nanoparticles was studied. Two-times amplification of gold nanoparticles was carried out to further improve the detection signal. It was suggested that the frequency shift of the indirect-labelling method was much more significant than that of the direct-labelling method. The detection limit of one-time amplification and two-times amplification detection of a 33-base oligonucleotide was 100 and 10 fM, respectively. Under corresponding sensitivity, the one-base mismatched DNA and complementary DNA could be distinguished clearly. As an example, the two-times amplification detection of 677TT gene type validated that the ratio of frequency shift for a complementary DNA: one-base-mismatched one was 2.1:1 under the detection limit of 10 fM.
机译：金纳米颗粒用作质量放大器，以改善QCM检测DNA的频率信号。研究了金纳米颗粒对DNA探针的间接标记和直接标记。金纳米颗粒进行了两次放大，以进一步改善检测信号。有人认为，间接标记法的频移比直接标记法的频移大得多。 33碱基的寡核苷酸的1次扩增和2次扩增检测的检出限分别为100fM和10fM。在相应的灵敏度下，可以清楚地区分一碱基错配的DNA和互补的DNA。例如，对677TT基因类型的两次扩增检测验证了互补DNA的频移比：一个碱基不匹配的碱基在10 fM的检测限下为2.1：1。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号