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Synthesis and characterization of europium(III) nanoparticles for time-resolved fluoroimmunoassay of prostate-specific antigen

机译：time（III）纳米粒子的合成和表征，用于前列腺特异性抗原的时间分辨荧光免疫测定

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摘要

Recent advances in the fabrication and bioconjugation of nanometre-sized lanthanide(III) chelate particles have led to robust high specific activity labels. This paper describes the synthesis and characterization of lanthanide(III) nanoparticle labels and the use of a nanoparticle in a bioaffinity assay system. Two europium(III) nanoparticles were prepared using an extremely simple, inexpensive and fast agglomeration strategy. A silica-stabilized nanoparticle was synthesized from hydrophobic tris(dibenzoylmethane)-mono(phenanthroline) and tris(dibenzoylmethane)-mono(5-aminophenanthroline) europium(III) chelates in aqueous solution. In addition, a naphthoyl trifluoroacetone: tri-n-octylphosphineoxide: sodium dodecyl sulfate europium(III) complex was agglomerated in water. The particle sizes ranged from 62 to 140 nm in diameter. The silica-stabilized particle was further coated with a monoclonal antibody. The analytical performance of the bioconjugated nanoparticle label was evaluated in a model sandwich immunoassay of prostate-specific antigen. The detection limit of human prostate-specific antigen was 28 ng l(-1), 850 fM, in a microtiter plate format using time-resolved fluorometry. The coefficient of variation ranged from 1 to 9%. The novel nanoparticle label improves the specific activity of existing lanthanide(III) nanoparticle labels and simplifies the preparation route. In addition, prepared high-density nanoparticle labels using lanthanide(III) chelates or other specific fluorochromes have potential applications in a number of other fields.
机译：纳米级镧系元素（III）螯合物颗粒的制备和生物共轭技术方面的最新进展已导致了强大的高比活标记物。本文介绍了镧系元素（III）纳米颗粒标记的合成和表征，以及纳米颗粒在生物亲和力测定系统中的用途。使用极其简单，廉价且快速的团聚策略制备了两种euro（III）纳米粒子。由水溶液中的疏水性三（二苯甲酰甲烷）-单（菲咯啉）和三（二苯甲酰甲烷）-单（5-氨基菲咯啉）euro（III）螯合物合成了二氧化硅稳定的纳米颗粒。另外，在水中团聚萘基三氟丙酮：三正辛基氧化膦：十二烷基硫酸钠sodium（III）络合物。粒径范围为直径62至140nm。二氧化硅稳定的颗粒进一步用单克隆抗体包被。在前列腺特异性抗原的模型夹心免疫分析中评估了生物共轭纳米颗粒标记的分析性能。使用时间分辨荧光法以微量滴定板形式检测人前列腺特异性抗原的检出限为28 ng l（-1），850 fM。变异系数在1％到9％之间。该新型纳米颗粒标记物提高了现有镧系元素（III）纳米颗粒标记物的比活性，并简化了制备途径。另外，使用镧系元素（III）螯合物或其他特定的荧光染料制备的高密度纳米颗粒标记物在许多其他领域中具有潜在的应用。

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