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Selective separation of proteins with pH-dependent magnetic nanoadsorbents

机译：用pH依赖的磁性纳米吸附剂选择性分离蛋白质

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摘要

Two kinds of unique pH-dependent magnetic nanoadsorbents based on silica coated magnetic nanoparticles (SMNPs) and amino-silica coated magnetic nanoparticles (ASMNPs) have been exploited for selective separation of proteins. With different isoelectric points, silica coated magnetic nanoadsorbents (SMNAs) and amino-silica coated magnetic nanoadsorbents (ASMNAs) can respectively adsorb proteins with different charges. The interactions between proteins and magnetic nanoadsorbents changed with the solution pH. Thus, the adsorption or desorption between proteins and magnetic nanoadsorbents can be controlled by changing the solution pH according to the charge of the proteins. And the magnetic nanoadsorbents can be separated and recycled simply with a magnet. As model adsorbates, Cyt-c and BSA are selectively separated from simple protein mixtures with SMNAs and ASMNAs respectively. Cyt-c was adsorbed on SMNAs at pH 8.0 at 0.160 mg mg~(-1). And BSA was adsorbed on ASMNAs at pH 5.0 at 0.142 mg mg~(-1).
机译：已经开发了两种独特的基于pH值的磁性纳米吸附剂，它们分别基于二氧化硅涂层的磁性纳米颗粒（SMNPs）和氨基二氧化硅涂层的磁性纳米颗粒（ASMNPs）进行蛋白质的选择性分离。具有不同等电点的二氧化硅包覆的磁性纳米吸附剂（SMNA）和氨基二氧化硅包覆的磁性纳米吸附剂（ASMNA）可以分别吸附具有不同电荷的蛋白质。蛋白质和磁性纳米吸附剂之间的相互作用随溶液pH的变化而变化。因此，可以通过根据蛋白质的电荷改变溶液的pH来控制蛋白质与磁性纳米吸附剂之间的吸附或解吸。磁性纳米吸附剂可以简单地用磁体分离和回收。作为模型吸附物，分别从具有SMNA和ASMNA的简单蛋白质混合物中选择性分离Cyt-c和BSA。 Cyt-c以0.160 mg mg〜（-1）吸附在pH 8.0的SMNAs上。 BSA以0.142 mg mg〜（-1）的pH 5.0吸附在ASMNAS上。

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