Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Rapid Detection of Isolates Belonging to the Epidemic Clones Achromobacter xylosoxidans ST137 and Achromobacter ruhlandii DES from Cystic Fibrosis Patients

Garrigos Thomas; Dollat Manon; Magallon ArnaudChapuis AngeliqueVarin VeroniqueBador JulienMakki NadiaCremet LisePersyn EliseCardot-Martin EmilieEchahidi FedouaPeeters CharlottePierard DenisVandamme PeterVerroken AlexiaNeuwirth CatherineAmoureux Lucie

首页> 外文期刊>Journal of Clinical Microbiology >Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Rapid Detection of Isolates Belonging to the Epidemic Clones Achromobacter xylosoxidans ST137 and Achromobacter ruhlandii DES from Cystic Fibrosis Patients

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Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Rapid Detection of Isolates Belonging to the Epidemic Clones Achromobacter xylosoxidans ST137 and Achromobacter ruhlandii DES from Cystic Fibrosis Patients

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Achromobacter spp. are increasingly reported among cystic fibrosis patients. Genotyping requires time-consuming methods such as multilocus sequence typing or pulsed-field gel electrophoresis. Therefore, data on the prevalence of multi-resistant epidemic clones, especially A. xylosoxidans ST137 (AxST137) and the Danish epidemic strain A. ruhlandii (DES), are lacking. We recently developed and published a database for Achromobacter species identification by matrix-assisted laser desorption-ionization-time of flight mass spectrometry (MALDI-TOF MS; Bruker Daltonics). The aim of this study was to evaluate the ability of the MALDI-TOF MS to distinguish these multiresistant epidemic clones within Achromobacter species. All the spectra of A. xylosoxidans (n =1,571) and A. ruhlandii (n =174) used to build the local database were analyzed by ClinProTools, MALDI Biotyper PCA, MALDI Biotyper dendrogram, and flexAnalysis software for biomarker peak detection. Two hundred two isolates (including 48 isolates of AxST137 and 7 of DES) were tested. Specific biomarker peaks were identified: absent peak at m/z 6,651 for AxST137 isolates and present peak at m/z 9,438 for DES isolates. All tested isolates were well typed by our local database and clustered within distinct groups (ST137 or non-ST137 and DES or non-DES) no matter the MALDI-TOF software or only by simple visual inspection of the spectra by any user. The use of MALDI-TOF MS allowed us to identify isolates of A. xylosoxidans belonging to the AxST137 clone that spread in France and Belgium (the Belgian epidemic clone) and of A. ruhlandii belonging to the DES clone. This tool will help the implementation of segregation measures to avoid interpatient transmission of these resistant clones.

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《Journal of Clinical Microbiology》 |2021年第10期|共8页
作者
Garrigos Thomas; Dollat Manon; Magallon ArnaudChapuis AngeliqueVarin VeroniqueBador JulienMakki NadiaCremet LisePersyn EliseCardot-Martin EmilieEchahidi FedouaPeeters CharlottePierard DenisVandamme PeterVerroken AlexiaNeuwirth CatherineAmoureux Lucie;
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Univ Hosp Dijon, Dept Bacteriol, Dijon, France;

CHU Nantes, Serv Bacteriol Hyg, Nantes, France;

Foch Hosp, Dept Microbiol, Suresnes, FranceVrije Univ Brussel, Univ Ziekenhuis Brussel, Dept Microbiol, Natl Reference Ctr BurkholderiaKatholieke Univ Leuven, Lab Clin Bacteriol & Mycol, Dept Microbiol & Immunol, Leuven, BelgiumUniv Ghent, Lab Microbiol, Natl Reference Ctr Burkholderia Cepacia Complex, Ghent, BelgiumCatholic Univ Louvain, Dept Microbiol, Clin Univ St Luc, Brussels, Belgium;

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正文语种英语
中图分类医学微生物学（病原细菌学、病原微生物学）;
关键词
Achromobacter xylosoxidans; Achromobacter ruhlandii; ST137; Danish epidemic strain; MALDI-TOF MS; typing; cystic fibrosis; epidemic clones;

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Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Rapid Detection of Isolates Belonging to the Epidemic Clones Achromobacter xylosoxidans ST137 and Achromobacter ruhlandii DES from Cystic Fibrosis Patients

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