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Free Radicals and Cell Chemiluminescence

机译：自由基和细胞化学发光

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摘要

Application of chemiluminescence (CL) for study of free-radical reactions in human and animal cells and tissues is considered in this review. Historically, the study of intrinsic (ultraweak) luminescence gave place to the measurement of CL in the presence of chemical activators (CL probes) and physical activators (sensitizers) of luminescence, which made the method much more sensitive and specific. The methods of CL and EPR are direct methods of radical investigation, though the advantage of the CL method consists in the fact that CL intensity is directly proportional to a steady-state concentration of the radicals responsible for luminescence (first of all, lipid and oxygen radicals) irrespective the activity of these radicals. The mechanisms of CL reactions in the absence of activators and in the presence of luminol and lucigenin are considered. Examples of various applications of the CL method in medical, biological, and clinical investigations are given including those for estimation of the phagocytic activity of cells, antioxidant activity, determination of toxicity, and other purposes.
机译：在这篇综述中考虑了化学发光（CL）在研究人类和动物细胞与组织中的自由基反应中的应用。从历史上看，在存在化学发光激活剂（CL探针）和物理发光激活剂（敏化剂）的情况下，固有（超弱）发光的研究被用于测量CL，这使该方法更加灵敏和特异。 CL和EPR的方法是自由基研究的直接方法，尽管CL方法的优点在于CL强度与负责发光的自由基的稳态浓度成正比（首先是脂质和氧气）自由基），而与这些自由基的活性无关。考虑了在不存在活化剂以及存在鲁米诺和光泽精的情况下CL反应的机制。给出了CL方法在医学，生物学和临床研究中的各种应用示例，包括那些用于估计细胞吞噬活性，抗氧化剂活性，毒性测定和其他目的的示例。

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