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首页> 外文期刊>Trauma >Effect of meniscus storage at 4 degrees C upon cell morphology and capacity to respond to growth factors TGF-beta 1, IGF-1, aFGF, bFGF and BMP-7 in monolayer culture
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Effect of meniscus storage at 4 degrees C upon cell morphology and capacity to respond to growth factors TGF-beta 1, IGF-1, aFGF, bFGF and BMP-7 in monolayer culture

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Aim: To evaluate the viability of meniscus storage at 4 degrees C. Material and method: Meniscus fragments were obtained from healthy goats under sterile conditions. As control, use was made of tissue fragments digested immediately after meniscus harvesting; the rest were stored immersed at 4 degrees C in culture medium for 15 and 30 days. Following storage, the material was digested using trypsin, collagenase and dispase, with monolayer culture of the cells. Cell morphology was evaluated under the light microscope, and cell response to growth factors TGF-beta 1, IGF-1, aFGF, bFGF and BMP-7 was assessed by proliferation tests. Results: The cell response to bFGF and IGF-1 increased after 15 days of treatment, followed by a decrease after 30 days. The response capacity after BMP-7 treatment decreased after 15 days and returned to control levels after 30 days. Factors aFGF and TGF-beta did not modify cell response. Conclusions: Following storage at 4 degrees C, the cells undergo morphological and biochemical changes that modify their sensitivity to certain growth factors.

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