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首页> 外文期刊>Trauma >Megalin is the receptor for caveola-mediated endocytosis of albumin and it's required for the synthesis of the neurotrophic factor oleic acid
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Megalin is the receptor for caveola-mediated endocytosis of albumin and it's required for the synthesis of the neurotrophic factor oleic acid

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Aim: Study of the endocytic pathway of albumin in rat astrocytes from primary culture. Material y Methods: One day postnatal Wistar rats were used to obtain astrocytes form primary culture. Astrocytes were incubated with 2 albumin or 0.5 FITC-albumin during 20 minutes at 4 degrees C for binding experiments or during 20 minutes at 4 degrees C followed by 10 minutes at 37 degrees C for internalization experiments. For loss-of-function experiments, astrocytes were transfected during 72h with 50 nM of siRNA specific for target protein, followed by the binding and internalization experiments. Oleic acid present in the culture medium of astrocytes incubated with 2 albumin during one hour was quantified by HPLC. Results: We observed that megalin and caveolin-1, but not clathrin, co-localize with albumin in the membrane. Megalin and caveolin-1 silencing by siRNA reduces albumin binding and internalization, as well as oleic acid synthesis in astrocytes. Nonetheless, clathrin silencing do not modify albumin internalization or oleic acid synthesis in astrocytes Conclusions: albumin is internalized in a caveola-dependent mechanism via megalin and that this event is required for the synthesis of the neurotrophic factor oleic acid.

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