...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Romanian Journal of Laboratory Medicine >Real-time qPCR for assessment of minimal residual disease in acute myeloid and lymphoid leukemia
【24h】

Real-time qPCR for assessment of minimal residual disease in acute myeloid and lymphoid leukemia

机译：

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Evaluation of minimal residual disease is of major importance in the course of acute leukemia treatment. There are a number of technologies used in this regard, all of them exhibiting different limitations. Real time qPCR is one of the main technologies enabling gene expression analysis and is well suited for minimal residual disease monitoring of acute leukemia patients. cDNA of eight most common fusion genes was cloned PML-RAR alpha, TEL-AML1, AML-ETO, E2A-PBX1, SIL-TAL1, CBF beta-MYH11, MLL-AF4, BCR-ABL1. Serial dilution of cloned plasmids were prepared and used as standards for real-time qPCR. Sensitivity and overall performance of the method was evaluated as previously described. Overall testing demonstrated robustness of TaqMan technology for gene expression analysis. High reproducibility with low levels of both inter and intra run variation was obtained. Sensitivity exhibited by the assay was comparable to nested PCR which allowed early relapse detection for a number of patients.

著录项

相似文献

  • 外文文献
  • 中文文献
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号