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首页> 外文期刊>Egyptian Journal of Basic and Applied Sciences >Genome-wide analysis and in silico screening of secondary metabolite potential of endophytic fungi Fusarium multiceps BPAL1 obtained in Mumbai, India
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Genome-wide analysis and in silico screening of secondary metabolite potential of endophytic fungi Fusarium multiceps BPAL1 obtained in Mumbai, India

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摘要

ABSTRACT Endophytic fungi are potent secondary metabolite producers due to their symbiotic existence. Mangrove environments offer diverse applications due to their rich diversity. The phytal ecosystem is a rich source of fungal isolates that exist as endogenous symbionts. Though the mangrove studies elaborate the medicinal values of the diverse plants and associated fungal species, limited information is available for species such as Fusarium sp. which have the potential to produce many secondary metabolites and bioactive compounds. Specific attention to carrying out a detailed study of genomic features, genetic information, and biosynthetic gene clusters (BGCs) enables the maximum utility of the byproducts of this species. The whole genome sequence of Fusarium multiceps BPAL1 (ON668124) strain isolated as an endophyte from Acanthus ilicifolius and gene annotation have been carried out during the current study. The assembled genome was found to be 39.57 Mb in size having a total of 169 scaffolds, and 47.62 GC content. Potential gene families crucial for biological processes were identified using Genome mining. antiSMASH analysis identified 33 biosynthetic gene clusters (BGCs) from 11,675 predicted genes, of which 23 clusters were found to be unknown. 11,309 genes that accounted for 96.87 of the candidate genes were annotated for their putative function/s. The overall predicted genes comprised 85.36 (9654) Eukaryotic Orthologous Groups and 28.41 (3213) involved in metabolism. De novo generated whole genome of Fusarium will be a good resource for future research while considering the expression, regulation, function, and evolution of Fusarium genes and their biotechnological applications.

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