Note: Raman microspectroscopy integrated with fluorescence and dark field imaging

Haibo Li; Hailong Wang; Dianshuai HuangLijia LiangYuejiao GuChongyang LiangShuping XuWeiqing Xu

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Note: Raman microspectroscopy integrated with fluorescence and dark field imaging

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A Raman detection platform integrated with both fluorescence and dark field microscopes was built for in situ Raman detection with the assistance of fluorescence and dark field imaging to locate the target micro regions. Cells and organelles can be easily found via fluorescence imaging with labeling techniques. Besides, nano-sized particles could be observed and located by dark field microscopes. Therefore, comparing with the commercial Raman spectrometers, much more researches based on Raman spectroscopy could be carried out on this integrated Raman platform, especially in the fields of analyzing biological tissues and subwavelength samples.

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来源
《Review of Scientific Instruments》 |2014年第5期|056109-1-056109-3|共3页
作者
Haibo Li; Hailong Wang; Dianshuai HuangLijia LiangYuejiao GuChongyang LiangShuping XuWeiqing Xu;
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作者单位

State Key Laboratory of Supramolecular Structure and Materials, Jilin University, Changchun 130012, People's Republic of China;

Institute of Frontier Medical Science, Jilin University, Changchun 130021, People's Republic of China;

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Note: Raman microspectroscopy integrated with fluorescence and dark field imaging

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