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首页> 外文期刊>North American Journal of Aquaculture >The Safety and Effectiveness of Various Hydrogen Peroxide and Iodine Treatment Regimens for Rainbow Trout Egg Disinfection
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The Safety and Effectiveness of Various Hydrogen Peroxide and Iodine Treatment Regimens for Rainbow Trout Egg Disinfection

机译:虹鳟卵消毒中各种过氧化氢和碘处理方案的安全性和有效性

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摘要

Four tests were conducted to evaluate iodine and hydrogen peroxide for the disinfection of rainbow trout Oncorhynchus mykiss eggs at higher doses for shorter durations than previously studied. In the first test, eyed eggs were exposed to (1) 2,000 mg iodine/L for 10 min, (2) 100 mg iodine/L for 15 min, (3) 30 g hydrogen peroxide/L for 1 min, (4) 6 g hydrogen peroxide/L for 5 min, or (5) no treatment. Iodine (2,000 mg/L) or hydrogen peroxide (30 g/L) significantly reduced bacterial loads on eggs but did not significantly affect egg survival or fry deformity rates. Hydrogen peroxide at 30 g/L for 1 min was generally better for bacterial control than the other treatments, but the 2,000-mg/L iodine treatment also was effective. A second test assessed the effect of hydrogen peroxide on pH at various levels of water hardness. The pH of hydrogen peroxide solutions dropped as total hardness levels decreased, but buffering with at least 1.32 g NaHCO3/L returned pH to approximately neutral levels. In the third test, in which eggs were treated 30 or 60 min postfertilization, there was no significant difference in survival between those treated with 15 g of buffered hydrogen peroxide/L for 2 min and that of the controls. However, at both 30 and 60 min postfertilization, the 2,000-mg/L iodine treatment induced higher levels of egg mortality than in eggs treated with hydrogen peroxide and the controls. In the fourth test, the serial combination of both 30 g hydrogen peroxide/L and 2,000 mg iodine/L was highly lethal if hydrogen peroxide was the first of the two treatments. The survival of eggs treated in the reverse order (iodine first) did not significantly differ from that of controls. These results indicate that hydrogen peroxide was effective in safely reducing the abundance of bacteria on eggs in small-scale tests when buffered, but production-scale experiments with hydrogen peroxide are recommended before implementation of this treatment.
机译:进行了四个测试,以评估碘和过氧化氢对虹鳟Onkihynchus mykiss卵的消毒作用,其剂量较高,持续时间比以前研究的时间短。在第一个测试中,将有眼卵暴露于(1)2,000 mg碘/ L 10分钟;(2)100 mg碘/ L 15分钟;(3)30 g过氧化氢/ L 1分钟;(4)每升6 g过氧化氢5分钟,或(5)不处理。碘(2,000 mg / L)或过氧化氢(30 g / L)显着降低了鸡蛋上的细菌载量,但并未显着影响鸡蛋的存活率或鱼苗畸形率。 30 g / L的过氧化氢1分钟通常比其他处理方法对细菌的控制效果更好,但是2,000 mg / L的碘处理也有效。第二项测试评估了过氧化氢在各种水硬度水平下对pH的影响。随着总硬度的降低,过氧化氢溶液的pH值下降,但是用至少1.32 g NaHCO3 / L缓冲可将pH值恢复到近似中性水平。在第三个试验中,在受精后30或60分钟对卵进行处理,用15 g缓冲过氧化氢/ L处理2 min的卵与对照组的存活率无显着差异。但是,在受精后30和60分钟,与用过氧化氢和对照处理的鸡蛋相比,用2,000 mg / L碘处理的鸡蛋死亡率更高。在第四项测试中,如果过氧化氢是两种处理方法中的第一项,则30 g过氧化氢/ L和2,000 mg碘/ L的系列组合具有很高的致死性。以相反顺序(先碘)处理的卵的存活率与对照的存活率没有显着差异。这些结果表明,过氧化氢在缓冲后的小规模试验中可有效安全地减少鸡蛋上的细菌数量,但建议在实施该处理之前使用过氧化氢进行生产规模的实验。

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