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首页> 外文期刊>North American Journal of Aquaculture >Fish Handling and Ultrasound Procedures for Viewing the Ovary of Submersed, Nonanesthetized, Unrestrained Channel Catfish
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Fish Handling and Ultrasound Procedures for Viewing the Ovary of Submersed, Nonanesthetized, Unrestrained Channel Catfish

机译：鱼类处理和超声检查程序，用于观察浸没的，非麻醉的，不受约束的Channel鱼的卵巢

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摘要

This study addressed the development of rapid, straightforward, and minimally stressful procedures for the ultrasound imaging of ovaries of channel catfish Ictalurus punctatus in a commercial hatchery setting. The objectives were to (1) describe the ultrasound imaging equipment and settings used, (2) describe the fish handling procedures during imaging, and (3) illustrate image orientation with respect to the physical positioning of the probe and the catfish. Ultrasound images of the ovaries of channel catfish were recorded as digital video recordings (audio video interleave format) and as still images (ultrasound image format files). This study integrated the use of nonanesthetized, submersed fish within a recirculating tank system or portable container and a submersed waterproof probe, which enabled us to use water as a transmission medium for ultrasound. This allowed us to image the fish in ventral recumbency (upright swimming position) without using a physical restraint in the tank system, or by positioning the fish in the portable container by adjusting the position of its caudal peduncle with one hand and that of the probe with the other hand. The ease of using this technique allows it to be employed as a systematic method for fish handling under laboratory and hatchery conditions. The detailed ultrasound imaging procedures and instrument control settings reported can be used in future testing, improvement, and standardization of procedures for viewing ovaries in channel catfish and potentially other species.
机译：这项研究致力于在商业孵化场设置快速，简单和压力最小的程序，用于对cat鱼I鱼卵巢进行超声成像。目的是（1）描述使用的超声成像设备和设置，（2）描述成像期间的鱼类处理程序，以及（3）说明相对于探头和the鱼的物理位置的图像方向。 channel鱼卵巢的超声图像记录为数字视频记录（音频视频交织格式）和静止图像（超声图像格式文件）。这项研究将非麻醉，浸入水中的鱼在循环水箱系统或便携式容器中的使用与浸入式防水探头整合在一起，这使我们能够将水用作超声波的传输介质。这使我们能够在腹侧卧位（直立游泳姿势）中对鱼进行成像，而无需在水箱系统中使用物理约束，也无需通过用一只手和探针调节尾柄的位置来将鱼放置在便携式容器中另一方面。该技术的易用性使其可以用作实验室和孵化场条件下鱼类处理的系统方法。报告的详细超声成像程序和仪器控制设置可用于将来的测试，改进和标准化程序，以查看海channel鱼和潜在其他物种的卵巢。

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