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首页> 外文期刊>Biochemistry >Stage Specific Developmental Changes in the Mitochondrial and Surface Membrane Associated Redox Systems of Leishmania donovani Promastigote and Amastigote
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Stage Specific Developmental Changes in the Mitochondrial and Surface Membrane Associated Redox Systems of Leishmania donovani Promastigote and Amastigote

机译：利什曼原虫和鞭毛体线粒体和表面膜相关氧化还原系统的阶段特定发育变化。

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Energy metabolism of Leishmania donovani parasite has been investigated under conditions imitating intralysoso-mal-like environment in the host organism. Trans-plasma membrane electron transport and oxygen uptake were inhibited progressively when promastigote cells were exposed to pH 5.5 and 37°C. A special feature of the respiratory chain in amastigote was the absence of complex I, II, and IV. When L. donovani was grown at pH 5.5 and 37°C, the acid excretory product succinate was increased in comparison to cells grown at pH 7.5 and 24°C. The findings of this study showed that the amastigote form catabolized fatty acid to excrete succinic acid when oxidative phosphorylation was impaired. Amastigote mitochondria failed to generate membrane potential by oxidizable substrates. On the other hand, the amastigote cell showed absorbance change of safranine O when fatty acid was the oxidizable substrate. The safranine signal was completely reversed by valinomycin, carbonyl cyanide 4-(trifluromethoxy)phenylhydrazone, malonate, and oxaloacetate. Our data suggest that the generation of metabolic energy from succinate/H~+ efflux will contribute to energy requiring process of amastigote significantly. On the basis of these results, we conclude that due to absence of oxidative phosphorylation in amastigotes, energy linked functions in amastigotes might occur through fumarate reduction leading to ApH generation by succinate excretion.
机译：已经在模拟宿主生物体内溶酶体样环境的条件下研究了多形利什曼原虫寄生虫的能量代谢。当前鞭毛体细胞暴露于pH 5.5和37°C时，跨质膜电子运输和氧气吸收被逐渐抑制。鞭毛体呼吸链的一个特殊特征是没有复合物I，II和IV。当多诺氏乳杆菌在pH 5.5和37°C下生长时，与在pH 7.5和24°C下生长的细胞相比，酸性排泄产物琥珀酸盐增加。这项研究的结果表明，当氧化磷酸化受损时，the蛇毒形式的脂肪酸分解代谢为琥珀酸。鞭毛线粒体不能通过可氧化的底物产生膜电位。另一方面，当脂肪酸为可氧化底物时，the蛇毒细胞显示出藏红素O的吸光度变化。番红霉素，羰基氰化物4-（三氟甲氧基）苯hydr，丙二酸酯和草酰乙酸完全逆转了藏红花信号。我们的数据表明，琥珀酸/ H〜+外排产生的代谢能将极大地促进鞭毛的能量需求。根据这些结果，我们得出结论，由于在无鞭毛体中不存在氧化磷酸化作用，所以通过富马酸酯的还原，琥珀酸的排泄会导致ApH的产生，而在无鞭毛体中可能发生能量相关的功能。

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