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Use of thermal lensing for the determination of pyrogens

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The conditions of spectrophotometric determination of lipopolysaccharides (LPS) by methylene blue dye were proposed. The limit of detection of LPS is 100 ng/ml. The conditions of spectrophotometric determination of LPS with Stains-all were proposed, the limit of detection of LPS is 500 ng/ml. The optimization of spectrophotometric determination of LPS by the reaction of 2-keto-3-deoxyoctonic acid with thiobarbituric acid in the presence of periodate and metaarsenite was made, the limit of detection is 800 ng/ml. The limit of detection by thermal lensing is 70 ng/ml. The thermal-lens method was successfully used for LPS determination in the endotoxin standard from E. coli. (C) 2003 American Institute of Physics. References: 21

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