Compact portable biosensor for arsenic detection in aqueous samples with Escherichia coli bioreporter cells

Frederic Truffer; Nina Buffi; Davide MerullaSiham BeggahHarald van LintelPhilippe RenaudJan Roelof van der MeerMartial Geiser

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Compact portable biosensor for arsenic detection in aqueous samples with Escherichia coli bioreporter cells

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We present a compact portable biosensor to measure arsenic As(III) concentrations in water using Escherichia coli bioreporter cells. Escherichia coli expresses green fluorescent protein in a linearly dependent manner as a function of the arsenic concentration (between 0 and 100 μg/L). The device accommodates a small polydimethylsiloxane microfluidic chip that holds the agarose-encapsulated bacteria, and a complete optical illumination/collection/detection system for automated quantitative fluorescence measurements. The device is capable of sampling water autonomously, controlling the whole measurement, storing and transmitting data over GSM networks. We demonstrate highly reproducible measurements of arsenic in drinking water at 10 and 50 μg/L within 100 and 80 min, respectively.

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《Review of Scientific Instruments》 |2014年第1期|015120-1-015120-4|共4页
作者
Frederic Truffer; Nina Buffi; Davide MerullaSiham BeggahHarald van LintelPhilippe RenaudJan Roelof van der MeerMartial Geiser;
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作者单位

Institute of Systems Engineering, University of Applied Sciences and Arts Western Switzerland Valais, Sion, Switzerland;

Laboratory of Microsystems Engineering, Ecole Polytechnique Federale Lausanne, EPFL-STI-LMIS, Station 17, CH-1015 Lausanne, Switzerland;

Department of Fundamental Microbiology, University of Lausanne, Switzerland;

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正文语种英语
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Compact portable biosensor for arsenic detection in aqueous samples with Escherichia coli bioreporter cells

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