Use of the comet assay to measure DNA damage in cells exposed to photosensitizers and gamma radiation

J.-P. Pouget; J.-L. Ravanat; T. DoukiM.-J. RichardJ. Cadet

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Use of the comet assay to measure DNA damage in cells exposed to photosensitizers and gamma radiation

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We used the comet assay associated with DNA-glycosylases to estimate DNA damage in cells exposed to gamma irradiation or photosensitized either with methylene blue or orange acridine. A calibration performed using #gamma# irradiation allowed the measurement of the steady-state level and the yield of 8-oxodGuo as well as strand breaks and alkali-labile sites.

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《Journal de Chimie Physique et de Physico-Chimie Biologique》 |1999年第1期|143-146|共4页
作者
J.-P. Pouget; J.-L. Ravanat; T. DoukiM.-J. RichardJ. Cadet;
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作者单位

Departement de Recherche Fondamentale sur la Matiere Condensee, SClB, Laboratoire Lesions des Acides Nucleiques, CEA Grenoble, 38054 Grenoble cedex 9, France;

LBSO/LCR7 No. 8M, Faculte de Pharmacie, Universite Joseph Fourier, 38700 La Tronche, France;

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正文语种英语
中图分类物理化学（理论化学）、化学物理学;
关键词
gamma; irradiation; DNA damages; photosensitization; comet assay;

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Use of the comet assay to measure DNA damage in cells exposed to photosensitizers and gamma radiation

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