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Enterocyte-like differentiation of the Caco-2 intestinal cell line is associated with increases in AP-1 protein binding

机译：Caco-2肠细胞系的肠样细胞分化与AP-1蛋白结合增加有关

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摘要

The nuclear factor AP-I, a large family of transcription factors composed of the Jun and Fos protein families, plays a role in the differentiation of various cells; the role of the AP-1 factors in intestinal differentiation is not known. Members of the AP-1 family can be activated by the Ras pathway and, in addition, Ras appears to be important for gut differentiation. The purpose of this study was to determine whether AP-1 activity is altered in the Caco-2 cell line, which spontaneously differentiates to a small bowel phenotype after confluency, and the Caco-2-ras cell line, which exhibits differentiated properties regardless of culture conditions. AP-1 binding activity, consisting of c-Jun, JunD, c-Fos and Fra-2 proteins, was increased in Caco-2 cells at 3 days postconfluency, a time point associated with G(1) block and cessation of proliferation. Steady state levels of JunD were increased at day 3 postconfluency as determined by Western blot. Furthermore, AP-1 binding was increased in preconfluent Caco-2-ras cells compared with parental Caco-2 cells, suggesting that AP-1 induction may be mediated by the Ras pathway. The early induction of AP-1 binding activity suggests a role for these proteins in the differentiation of the Caco-2 intestinal cell line. [References: 26]
机译：核因子AP-I是由Jun和Fos蛋白家族组成的大转录因子家族，在多种细胞的分化中发挥作用。 AP-1因子在肠道分化中的作用尚不清楚。 AP-1家族的成员可以被Ras途径激活，此外，Ras对于肠道分化也很重要。这项研究的目的是确定在融合后自发分化为小肠表型的Caco-2细胞系和Caco-2-ras细胞系是否具有AP-1活性变化，而无论文化条件。 AP-1结合活性，由c-Jun，JunD，c-Fos和Fra-2蛋白质组成，在融合后3天（与G（1）阻滞和停止增殖有关的一个时间点）在Caco-2细胞中增加。通过Western印迹测定，在汇合后第3天，JunD的稳态水平增加。此外，与亲本Caco-2细胞相比，融合前的Caco-2-ras细胞中AP-1的结合增加，表明AP-1的诱导可能是由R​​as途径介导的。 AP-1结合活性的早期诱导表明这些蛋白质在Caco-2肠细胞系分化中的作用。 [参考：26]

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