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The effect of periphyton stoichiometry and light on biological phosphorus immobilization and release in streams

机译:浮游植物化学计量学和光照对生物磷固定化和在流中释放的影响

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Periphyton stoichiometry can vary substantially as a result of differences in stream nutrient availability. A decrease in the periphyton carbon to phosphorus (C:P) ratio should decrease the demand for new P to be immobilized from stream water, but no studies to our knowledge have explored the relationship between periphyton stoichiometry and net P immobilization and release by periphyton. We sought to model biological P immobilization and release (flux) in streams by measuring periphyton stoichiometry and light availability. We measured P flux to and from intact periphyton on stream cobbles (20-100 mm diameter) in 1 L microcosms incubated with streamwater under variable light conditions. Net P immobilization occurred in 75% of microcosms, net P release occurred in only 5% of microcosms, and 20% of microcosms had neither net immobilization nor net release. When normalized to stream conditions, net P immobilization was highest when light availability was high (<60% canopy attenuation) and the periphyton C:P ratio was also high. In contrast, net P release occurred only when light availability was low (>60% canopy attenuation) and the periphyton C:P ratio was also low. A multiple regression model that included both periphyton stoichiometry and light availability from the growing season only, and the interaction term of these two variables, explained 99% of the variation in daily periphyton P flux observed in the study. These results indicate that in order to predict periphyton P immobilization, periphyton stoichiometry and light availability should be considered together. Furthermore, the results indicate that net P immobilization occurs even in very P-rich periphyton, which can act as a P sink when light availability is high.
机译:由于溪流养分利用率的差异,周生藻的化学计量可能会有很大变化。浮游植物碳磷比(C:P)的降低应减少对从河水中固定新磷的需求,但是据我们所知,没有研究探索浮游生物化学计量与净磷固定化和浮游植物释放之间的关系。我们试图通过测量周围植物的化学计量和光利用率来模拟流中生物P的固定和释放(通量)。我们测量了在可变光照条件下与溪流一起温育的1 L微观世界中,溪流卵石(直径20-100 mm)上完整的浮游植物的磷通量。净P固定发生在75%的微观世界中,净P释放仅发生在5%的微观世界中,而20%的微观世界既没有净固定也没有净释放。当归一化为河流条件时,当光利用率高(<60%的冠层衰减)并且周生植物的C:P比也很高时,净磷固定化最高。相比之下,净磷释放仅在光利用率低(> 60%的冠层衰减)且周生植物C:P比率也低时发生。多元回归模型仅包括生长季的浮游植物化学计量和光利用率,以及这两个变量的相互作用项,解释了研究中观察到的每日浮游植物P通量变化的99%。这些结果表明,为了预测周生植物P的固定化,应综合考虑周生植物的化学计量和光利用率。此外,结果表明,即使在非常富P的附生植物中也发生了净P固定化,当光利用率高时,它可以充当P吸收体。

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