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首页> 外文期刊>Redox report: communications in free radical research >Gene expression analysis following hypoxia-reoxygenation in rat gastric epithelial cells using a high-density oligonucleotide array
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Gene expression analysis following hypoxia-reoxygenation in rat gastric epithelial cells using a high-density oligonucleotide array

机译：使用高密度寡核苷酸阵列分析大鼠胃上皮细胞缺氧-复氧后的基因表达

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摘要

Recent investigations have demonstrated that the signaling of hypoxia-re-oxygenation is a major contributing pathway leading to gastric mucosal injury induced by stress, non-steroidal anti-inflammatory drugs, and Helicobacter pylori. The aim of the present study was to perform a gene expression analysis on the gastric mucosal cellular response to hypoxia–reoxygenation using a high-density oligonucleotide array. Cells were subjected to hypoxia with 95% N_2 and 5% CO_2 at 37 ℃ for 2 h. Reoxygenation was initiated by placing the cells in an environment of normoxia for 2 h. Total RNA was extracted, and differences in gene expression profiles between the normoxia and hypoxia-reoxygenation groups were investigated using a GeneChip of Rat Toxicology U34 array (Affymetrix). Hypoxia-reoxygenation up-regulated the stress-related genes (heat shock protein-70 [HSP-70], catalase). The enhanced expression of HSP-70 was confirmed by Western blot analysis. In conclusion, these results suggest that up-regulation of the HSP-70 gene after reoxygenation may play a role in maintaining cell survival and supporting cell function as a molecular chaperone.
机译：最近的研究表明，缺氧-再充氧的信号传导是导致压力，非甾体类抗炎药和幽门螺杆菌引起的胃粘膜损伤的主要促成途径。本研究的目的是使用高密度寡核苷酸阵列对胃粘膜细胞对缺氧-复氧的反应进行基因表达分析。在37℃下用95％N_2和5％CO_2的细胞进行缺氧2 h。通过将细胞置于常氧环境中2小时来开始进行复氧。提取总RNA，并使用大鼠毒理学U34阵列（Affymetrix）的GeneChip研究常氧和低氧-复氧组之间基因表达谱的差异。缺氧复氧上调了与压力相关的基因（热激蛋白70 [HSP-70]，过氧化氢酶）。通过蛋白质印迹分析证实了HSP-70的表达增强。总之，这些结果表明，复氧后HSP-70基因的上调可能在维持细胞存活和支持细胞功能上起分子伴侣的作用。

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