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首页> 外文期刊>Cellular immunology >Filament-associated TSGA10 protein is expressed in professional antigen presenting cells and interacts with vimentin.
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Filament-associated TSGA10 protein is expressed in professional antigen presenting cells and interacts with vimentin.

机译：与细丝相关的TSGA10蛋白在专业抗原呈递细胞中表达，并与波形蛋白相互作用。

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摘要

Testis-specific gene antigen 10 (TSGA10) encodes an 82-kDa protein expressed during development, and in testis and brain tissues. We report its expression in human monocyte-derived dendritic cells (DC) and macrophages in vitro and in murine spleen CD11c(+) cells ex vivo. An interaction between DC/macrophage-derived TSGA10 and vimentin, as well as a few other major cytoskeletal proteins (e.g., actin-gamma1), was identified by pull-down and mass spectroscopy assays. The interaction between TSGA10 and vimentin was further confirmed by immunoprecipitation and immunolocalisation in transfected RAW267 and HEK293 cell lines. TSGA10 formed filamentous structures in transfected COS-1 cells and was observed in cellular projections. We propose that TSGA10 could influence the function of antigen presenting cells (APC) via its interaction with cytoskeletal proteins such as vimentin.
机译：睾丸特异性基因抗原10（TSGA10）编码在发育期间以及在睾丸和脑组织中表达的82 kDa蛋白。我们报告其表达在人类单核细胞衍生的树突状细胞（DC）和巨噬细胞在体外和小鼠脾CD11c（+）细胞离体。 DC /巨噬细胞衍生的TSGA10与波形蛋白以及其他一些主要的细胞骨架蛋白（例如肌动蛋白-γ1）之间的相互作用通过下拉分析和质谱分析确定。通过在转染的RAW267和HEK293细胞系中进行免疫沉淀和免疫定位，进一步证实了TSGA10与波形蛋白之间的相互作用。 TSGA10在转染的COS-1细胞中形成丝状结构，并在细胞投影中观察到。我们建议TSGA10可以通过其与细胞骨架蛋白（例如波形蛋白）的相互作用来影响抗原呈递细胞（APC）的功能。

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