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Headspace measurements of irradiated in vitro cultured cells using PTR-MS

机译：使用PTR-MS测量辐照的体外培养细胞的顶空

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摘要

A pilot study was performed to evaluate a new concept for a radiation biodosimetry method. Proton transfer reaction-mass spectrometry (PTR-MS) was used to find out whether radiation induces changes in the composition of volatile organic compounds (VOCs) in the headspace of in vitro cultured cells. Two different cell lines, retinal pigment epithelium cells hTERT-RPE1 and lung epithelium cells A-549, were irradiated with gamma radiation at doses of 4 Gy and 8 Gy. For measuring the cell-specific effects, the VOC concentrations in the headspace of flasks containing cells plus medium, as well as of flasks containing pure medium were analyzed for changes before and after irradiation. No significant radiation-induced alterations in VOC concentrations in the headspace could be observed after irradiation.
机译：进行了一项初步研究，以评估辐射生物剂量法的新概念。质子转移反应质谱法（PTR-MS）用于确定辐射是否会诱导体外培养细胞顶部空间中挥发性有机化合物（VOC）的组成发生变化。两种不同的细胞系，即视网膜色素上皮细胞hTERT-RPE1和肺上皮细胞A-549，分别以4 Gy和8 Gy的剂量进行了伽玛射线照射。为了测量细胞特异性效应，分析了含有细胞和培养基的烧瓶以及含有纯培养基的烧瓶的顶部空间中VOC的浓度，以测定辐照前后的变化。辐照后未观察到顶空中VOC浓度的明显辐射诱导变化。

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