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首页> 外文期刊>Russian Journal of Physical Chemistry >Interaction of DNA with a poly-N-vinylpyrrolidone containing a terminal amino group and with its star-shaped fullerene derivative
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Interaction of DNA with a poly-N-vinylpyrrolidone containing a terminal amino group and with its star-shaped fullerene derivative

机译：DNA与含有末端氨基的聚N-乙烯基吡咯烷酮及其星形富勒烯衍生物的相互作用

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摘要

Aqueous saline solutions of DNA in the presence of poly-N-vinylpyrroli done (PVP), poly-N-vinylpyrrolidone containing a terminal amino group (PVP-NH2), and a star-shaped polymer (PVPstar) formed via the covalent bonding of PVP-NH2 chains to a fullerent, C-60 core (PVP-NH-C-60) were studied using hydrodynamic and optical methods. The concentration of NaClin the solution was 0.005 and 0.15M, while the molar ratio r of the number of PVP monomeric units to that of nucleotide pairs of DNA was varied from 0.5 to 10. Viscometry measurements demonstrated that these polymers interact with DNA to form complexes with a helical structure more compact than that of the free DNA molecule. The strongest effect of shrinkage of the DNA was produced by PVPstar. The specific volume of the solute particles decreased gradually and uniformly with increasing r at r <= 1 (for DNA + PVPstar complexes, by half in the 0.005 M NaCl, and by a factor of 1.25 in the 0.15 M NaCl), remaining constant at higher r within 1 <= r <= 10. The circular dichroism spectra and persistence length of DNA were found to independent of r. Various models of the DNA interaction with the polymers were discussed.
机译：DNA的盐水溶液在存在聚N-乙烯基吡咯烷酮（PVP），含有末端氨基的聚N-乙烯基吡咯烷酮（PVP-NH2）和星形聚合物（PVPstar）的情况下通过共价键形成使用流体动力学和光学方法研究了连接至完整C-60核（PVP-NH-C-60）的PVP-NH2链。溶液的NaClin浓度为0.005和0.15M，而PVP单体单元数与DNA核苷酸对的摩尔比r在0.5到10之间变化。粘度测量表明，这些聚合物与DNA相互作用形成复合物具有比游离DNA分子更紧密的螺旋结构。 DNA收缩的最强作用是由PVPstar产生的。在r <= 1时，溶质颗粒的比体积随着r的增加而逐渐均匀地减小（对于DNA + PVPstar复合物，在0.005 M NaCl中减小一半，在0.15 M NaCl中减小1.25倍），在r在1 <= r <= 10内更高。发现圆二色性光谱和DNA的持久长度与r无关。讨论了DNA与聚合物相互作用的各种模型。

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