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首页> 外文期刊>Oncology letters >Application of surface-enhanced laser desorption/ionization time-of-fight mass spectrometry technology for the diagnosis of colorectal adenoma
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Application of surface-enhanced laser desorption/ionization time-of-fight mass spectrometry technology for the diagnosis of colorectal adenoma

机译：表面增强激光解吸/电离飞行时间质谱技术在大肠腺瘤诊断中的应用

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摘要

The aim of the present study was to identify a specific biological marker for the diagnosis of colorectal adenomas through the analysis of variations in serum protein profiling in colorectal adenoma patients. The study was conducted at the Renmin Hospital of Wuhan University (Wuhan, China) between September 2011 and May 2012. Surface-enhanced laser desorption/ionization time-of-fight mass spectrometry (SELDI-TOF-MS) was performed to compare the serum protein profiles of 50 patients with colorectal adenoma and 50 healthy individuals. The obtained protein profiles were analyzed using Biomarker Wizard software. Twenty protein peaks were identified to exhibit differences in average intensity between colorectal adenomas compared with normal controls, including peaks 8,565.84, 8,694.51 and 5,910.50 Da, in which the intensity between the patients and control individuals was significantly different. Two peaks, 8,565.84 and 8,694.51 Da, were observed to be highly expressed in the colorectal adenomas, however, expression was low in the control samples. By contrast, 5,910.50 Da expression was low in the colorectal adenomas and high in the controls. The results of the current study indicate that the three protein peaks may represent specific biomarkers for colorectal adenomas.
机译：本研究的目的是通过分析大肠腺瘤患者血清蛋白谱的变化来鉴定诊断大肠腺瘤的特定生物学标志物。该研究于2011年9月至2012年5月在武汉大学人民医院（中国武汉）进行。采用表面增强激光解吸/电离飞行时间质谱（SELDI-TOF-MS）来比较血清50位大肠腺瘤患者和50位健康个体的蛋白质谱。使用Biomarker Wizard软件分析获得的蛋白质谱。与正常对照组相比，鉴定出二十个蛋白质峰显示出大肠腺瘤之间平均强度的差异，包括峰8,565.84、8,694.51和5,910.50 Da，其中患者和对照组个体之间的强度显着不同。观察到在结肠直肠腺瘤中高表达两个峰8568.54和8695.41 Da，但是在对照样品中表达较低。相比之下，5,910.50 Da的表达在结直肠腺瘤中较低，而在对照中则较高。目前的研究结果表明，这三个蛋白峰可能代表了结肠直肠腺瘤的特定生物标志物。

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