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Single-photon-counting detector for increased sensitivity in two-photon laser scanning microscopy

机译:单光子计数检测器可提高双光子激光扫描显微镜的灵敏度

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We present the use and characterization of a photon-counting detector for increased sensitivity at low signal levels in fluorescence laser scanning microscopy (LSM). Conventional LSM photomultiplier tube detectors utilize analog current integration and thus suffer from excessive noise at low signal levels, introduced during current measurement. In this Letter we describe the implementation of a fast single-photon-counting (SPC) detector on a conventional two-photon laser scanning microscope and detail its use in imaging low fluorescence intensities. We show that for a low photon flux, the SPC detector is shot-noise limited and thus provides increased detection sensitivity compared with analog current integration.
机译:我们目前使用和表征的光子计数检测器在荧光激光扫描显微镜(LSM)中在低信号水平下提高灵敏度。常规的LSM光电倍增管检测器利用模拟电流积分,因此在电流测量期间引入的低信号电平下会产生过多的噪声。在这封信中,我们描述了在传统的双光子激光扫描显微镜上快速单光子计数(SPC)检测器的实现方式,并详细介绍了其在低荧光强度成像中的用途。我们表明,对于低光子通量,SPC检测器受散粒噪声限制,因此与模拟电流积分相比,可提供更高的检测灵敏度。

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