Gene transfer into mammalian cells by use of a nanosecond pulsed laser-induced stress wave

Mitsuhiro Terakawa; Makoto Ogura; Shunichi Sato; Hitoshi Wakisaka; Hiroshi Ashida; Maki Uenoyama; Yoshinori Masaki; Minoru Obara

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Gene transfer into mammalian cells by use of a nanosecond pulsed laser-induced stress wave

机译：利用纳秒脉冲激光诱导的应力波将基因转移到哺乳动物细胞中

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摘要

Plasmid DNA has been successfully delivered to mammalian cells by applying a nanosecond pulsed laser-induced stress wave (LISW). Cells exposed to a LISW were selectively transfected with plasmids coding for green fluorescent protein. It was also shown that transient, mild cellular heating (～43℃) was effective in improving the transfection efficiency.

机译：通过施加纳秒脉冲激光诱导的应力波（LISW），质粒DNA已成功传递到哺乳动物细胞。暴露于LISW的细胞用编码绿色荧光蛋白的质粒选择性转染。研究还表明，短暂，温和的细胞加热（〜43℃）可以有效提高转染效率。

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《Optics Letters》 |2004年第11期|共3页
作者
Mitsuhiro Terakawa; Makoto Ogura; Shunichi Sato; Hitoshi Wakisaka; Hiroshi Ashida; Maki Uenoyama; Yoshinori Masaki; Minoru Obara;
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正文语种 eng
中图分类光学;
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机译：利用纳秒脉冲激光诱导的应力波将基因转移到哺乳动物细胞中：勘误
2. Targeted gene transfer into rat facial muscles by nanosecond pulsed laser-induced stress waves [J] . Akihiro Kurita, Takeshi Matsunobu, Yasushi Satoh, Journal of biomedical optics . 2011,第9期

机译：纳秒脉冲激光诱导的应力波将靶向基因转移到大鼠面部肌肉
3. Efficient delivery of small interfering RNA to plant cells by a nanosecond pulsed laser-induced stress wave for posttranscriptional gene silencing [J] . Tang W, Weidner DA, Hu BY, Plant Science: An International Journal of Experimental Plant Biology . 2006,第3期

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4. Gene transfer of human Hepatocyte Growth Factor by the use of nanosecond pulsed laser-induced stress waves [C] . Mitsuhiro Terakawa, Shunichi Sato, Daizoh Saitoh, High-Power Laser Ablation VI pt.2 . 2006

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5. Biophotonic studies of mammalian cells with nanosecond pulsed power and fluorescent quantum dots [D] . Sun, Yinghua 2006

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Gene transfer into mammalian cells by use of a nanosecond pulsed laser-induced stress wave

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