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Time-dependent changes in adhesive force between chondrocytes and silk fibroin substrate

机译：软骨细胞与丝素蛋白底物之间粘附力的时间依赖性变化

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In tissue engineering for cartilage repair using scaffold, initial chondrocyte-material interactions are significantly important for the following cell behaviors such as phenotypic expression and matrix synthesis. Silk fibroin scaffold is considered to be one of the useful materials in/on which chondrocytes can proliferate without dedifferentiating into fibroblast-like cells and can organize a hyaline-like tissue. For the purpose of seeking some useful aspects for designing scaffold, initial adhesive force of chondrocytes to the surface of fibroin substrate was measured by using a lab-made apparatus applying the cantilever beam method. It was found that the adhesive force per unit spreading area of chondrocytes on fibroin substrate had a clear peak between 6 and 12 h after seeding. From the results of immunofluorescence staining for actin and vinculin during this period, it could be thought that an immature formation of actin fibers which was uniquely observed at the periphery of cells attaching to fibroin substrate did not contribute to the increase of adhesive force, Results in this study suggested that surface of the fibroin substrate was gradually covered with some substances which inhibit the adhesion during this period. These cell-material interactions have a possibility to be useful information for designing the adhesive performance of scaffold surface in cartilage regeneration. (c) 2006 Published by Elsevier Ltd.
机译：在使用支架进行软骨修复的组织工程中，初始软骨细胞-材料相互作用对于随后的细胞行为（例如表型表达和基质合成）非常重要。丝素蛋白支架被认为是有用的材料之一，软骨细胞可在其中/上增殖而不会去分化为成纤维细胞样细胞，并可组织透明样组织。为了寻求一些设计支架的有用方面，通过使用悬臂梁法的实验室制造的设备来测量软骨细胞对纤维蛋白底物表面的初始粘附力。发现在播种后6至12小时之间，软骨细胞在纤维蛋白底物上的每单位扩散面积的粘附力具有清晰的峰值。从这一时期肌动蛋白和纽蛋白的免疫荧光染色结果来看，可以认为在粘附于丝蛋白底物的细胞周围独特观察到的肌动蛋白纤维的不成熟形成并没有促进粘附力的增加。这项研究表明，在此期间，纤维蛋白底物的表面逐渐被一些抑制粘附的物质覆盖。这些细胞-材料相互作用可能是有用的信息，可用于设计支架表面在软骨再生中的粘合性能。 （c）2006年由Elsevier Ltd.发布。

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