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首页> 外文期刊>Science in China, Series C. Life science >Effects of ELF magnetic fields on protein expression profile of human breast cancer cell MCF7
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Effects of ELF magnetic fields on protein expression profile of human breast cancer cell MCF7

机译：ELF磁场对人乳腺癌细胞MCF7蛋白表达谱的影响

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摘要

Extremely Low Frequency Magnetic Fields (ELF MF) has been considered as a "possible human carcinogen" by International Agency for Research on Cancer (IARC) while credible mechanisms of its carcinogenicity remain unknown. In this study, a proteomics approach was employed to investigate the changes of protein expression profile induced by ELF MF in human breast cancer cell line MCF7, in order to determine ELF MF-responsive proteins. MCF7 cells were exposed to 50 Hz, 0.4 mT ELF MF for 24 h and the changes of protein profile were examined using two dimensional electrophoresis. Up to 6 spots have been statistically significantly altered (their expression levels were changed at least 5 fold up or down) compared with sham-exposed group. 19 ones were only detected in exposure group while 19 ones were missing. Three proteins were identified by LC-IT Tandem MS as RNA binding protein regulatory subunit, Proteasome subunit beta type 7 precursor and Translationally Controlled Tumor Protein. Our finding showed that 50 Hz, 0.4 mT ELF MF alternates the protein profile of MCF7 cell and may affect many physiological functions of normal cell and 2-DE coupled with MS is a promising approach to elucidating cellular effects of electromagnetic fields.
机译：国际癌症研究机构（IARC）已将极低频磁场（ELF MF）视为“可能的人类致癌物”，但其致癌性的可靠机理仍不清楚。在这项研究中，采用蛋白质组学方法来研究ELF MF诱导的人乳腺癌细胞MCF7中蛋白质表达谱的变化，以确定ELF MF反应蛋白。将MCF7细胞暴露于50 Hz，0.4 mT ELF MF中24 h，并使用二维电泳检查蛋白质谱的变化。与假手术组相比，多达6个斑点在统计学上有显着改变（其表达水平至少上下改变了5倍）。仅在暴露组中检测到19个，而缺少19个。通过LC-IT串联质谱鉴定出了三种蛋白，它们分别是RNA结合蛋白调节亚基，蛋白酶体亚基β7型前体和翻译控制的肿瘤蛋白。我们的发现表明，50 Hz，0.4 mT ELF MF会改变MCF7细胞的蛋白质谱，并可能影响正常细胞的许多生理功能，而2-DE与MS结合是阐明电磁场对细胞的作用的有前途的方法。

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