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首页> 外文期刊>Separation and Purification Technology >Preparative isolation and purification of two flavonoid glycosides from Taraxacum mongolicum by high-speed counter-current chromatography
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Preparative isolation and purification of two flavonoid glycosides from Taraxacum mongolicum by high-speed counter-current chromatography

机译：高速逆流色谱法从蒙古蒲公英中制备和分离两种黄酮苷

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摘要

Two flavonoid glycosides,alquds and hesperidin were isolated and purified from the aerial part of Taraxacum mongolicum,a traditional Chinese medicine (TCM) by high-speed counter-current chromatography (HSCCC).After Qg open column chromatography served as enrichment step,high-speed counter-current chromatography (HSCCC) was used for the final purification.The target compounds were finally isolated and purified with a solvent system composed of n-hexane-n-butanol-water (1:1:2,v/v/v).In a single operation,0.6 g of the enriched extracts of T.mongolicum was separated to yield 84.6 mg of alquds and 45.1 mg of hesperidin with the purity of 98.8% and 98.1%,respectively.The HSCCC fractions were analyzed by high-performance liquid chromatography (HPLC) and the chemical structures of the two flavonoid glycosides were identified by UV,ESI-MS,1H NMR,13C NMR and HMBC analysis.Alquds and hesperidin were obtained from the plant of genus Taraxacum for the first time,which may be used for in vitro and in vivo studies and reference substances for analytical purpose.This technique should also be applicable to the preparation of unknown bioactive compounds from traditional Chinese medicine (TCM).
机译：通过高速逆流色谱（HSCCC）从中药蒲公英（TCM）的地上部分中分离纯化出两种黄酮苷，水苷和橙皮苷。高速逆流色谱法（HSCCC）进行最终纯化，最后分离目标化合物，并用正己烷-正丁醇-水（1：1：2，v / v / v）组成的溶剂系统进行纯化）。在一次操作中，分离出0.6 g的蒙古樟脑提取物，分别制得84.6 mg的alqus和45.1 mg的橙皮苷，其纯度分别为98.8％和98.1％。用高效液相色谱（HPLC）进行鉴定，并通过UV，ESI-MS，1H NMR，13C NMR和HMBC分析鉴定了两种类黄酮苷的化学结构。首次从蒲公英属植物中获得了水和橙皮苷。可用于体外和非体内研究和用于分析目的的参考物质。该技术也应适用于从中药（TCM）制备未知的生物活性化合物。

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