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Chlorophyll fluorescence: a new technique to screen for tolerance of strawberry flowers to spring frost

机译:叶绿素荧光:一种筛选草莓花对春季霜冻耐受性的新技术

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摘要

Most strawberry cultivars have flowers that are sensitive to temperatures below 0 deg C. The development of early or very early cultivars with frost resistant flowers is essential in climates with a danger of spring frosts. Traditionally, breeding programs have used visual screening methods to evaluate the damage to pistils and anthers caused by frost. These methods rely on natural seasonal conditions, are time consuming, and do not provide accurate information on the exact temperature that causedthe damage. The objective of this study was to evaluate the use of chlorophyll fluorescence (CF) to estimate the low temperature susceptibility of 64 strawberry cultivars. Strawberry flowers were exposed to continuous low temperatures (0 deg C for 24 h,-1 deg C for 24 h, -2 deg C for 24 h, and finally -3 deg C for 24 h) and CF was measured following the treatments. Variable fluorescence (Fv) decreased somewhat with time in all genotypes when the flowers were held at -3 deg C. however, the reduction varied with cultivar. The slight reduction of Fv in the more chilling-tolerant cultivars was not significant, while significant linear or quadratic declines were observed in the more chilling susceptible cultivars. Overall, chlorophyll fluorescence appearsto be an effective, simple method for evaluating the low temperature susceptibility of strawberry genotypes.
机译:大多数草莓品种的花都对低于0摄氏度的温度敏感。在具有春季霜冻危险的气候中,培育具有抗冻花的早期或非常早期的品种至关重要。传统上,育种计划使用视觉筛选方法来评估霜冻对雌蕊和花药的损害。这些方法依赖于自然的季节性条件,非常耗时,并且无法提供导致损坏的确切温度的准确信息。这项研究的目的是评估使用叶绿素荧光(CF)评估64个草莓品种的低温敏感性。将草莓花暴露于连续的低温下(0℃持续24 h,-1℃持续24 h,-2℃持续24 h,最后-3℃持续24 h),并在处理后测量CF。当将花保持在-3℃时,所有基因型的可变荧光(Fv)随时间略有下降,但是,下降随品种而变化。耐冷性更强的品种中Fv的轻微降低并不显着,而耐冷性更强的品种中则观察到显着的线性或二次下降。总体而言,叶绿素荧光似乎是评估草莓基因型低温敏感性的一种有效,简单的方法。

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