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首页> 外文期刊>Biological chemistry >Internalisation and Degradation of Histatin 5 by Candida albicans
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Internalisation and Degradation of Histatin 5 by Candida albicans

机译：白色念珠菌对组蛋白5的内在化和降解

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Histatins, salivary antimicrobial peptides, are susceptible to proteolytic degradation, often ascribed to host proteinases. In this study, we addressed the question whether proteolytic activity from microbial sources can contribute to this degradation. Candida albicans, an opportunistic yeast that is susceptible to the histatins, was used as target organism. The most potent histatin (histatin 5: sequence: DSHAKRHH-GYKRKFHEKHHSHRGY), two histatin 5 fragments (dh-5: sequence: KRKFHEKHHSHRGY; P-113: sequence: AKRHHGYKRKFH) and an all-D isomer of the latter (P-113D) were used as model peptides. All L-peptides were susceptible to degradation by C. albicans. Cleavage was established at Lys5 and His19 of histatin 5, Lys11, Arg12, Phe14, Glu16, Lys17, His18 and Ser20 of dh-5 and Ala4 and Lys11 of P-113. In addition, it was found that secreted C. albicans enzymes are not involved in the degradation process and that blocking cell entry of the peptides greatly impedes degradation. Moreover, P-113D, which is biologically as active as P-113, was hardly susceptible to proteolysis. These data imply that proteolysis occurs mainly intracellularly and is not used as a protective mechanism against histatin activity. Together, our results suggest that, besides host proteinases, microbial enzymes play an important role in histatin degradation.
机译：唾液抗菌肽Histatins易发生蛋白水解降解，通常归因于宿主蛋白酶。在这项研究中，我们解决了以下问题：微生物来源的蛋白水解活性是否会导致这种降解。白色念珠菌是一种对组蛋白敏感的机会酵母，被用作目标生物。最有效的组蛋白（组蛋白5：序列：DSHAKRHH-GYKRKFHEKHHSHRGY），两个组蛋白5片段（dh-5：序列：KRKFHEKHHSHRGY; P-113：序列：AKRHHGYKRKFH）和后者的全D异构体（P-113D）被用作模型肽。所有的L肽都容易被白色念珠菌降解。切割建立在组蛋白5的Lys5和His19，Lys11，Arg12，Phe14，Glu16，Lys17，His18和Ser20的dh-5和A-la4和Lys11的P-113上。另外，发现分泌的白色念珠菌酶不参与降解过程，并且阻断肽的细胞进入极大地阻碍了降解。而且，与P-113具有生物学活性的P-113D几乎不易被蛋白水解。这些数据暗示蛋白水解主要发生在细胞内，并且不被用作针对组蛋白活性的保护机制。总之，我们的结果表明，除宿主蛋白酶外，微生物酶在组蛋白降解中也起着重要作用。

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