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首页> 外文期刊>Spectrochimica acta, Part A. Molecular and biomolecular spectroscopy >Interaction of cyanine dyes with nucleic acids. XXV. Influence of affinity-modifying groups in the structure of benzothiazol-4-[2,6-dimethylpyridinium] dyes on the spectral properties of the dyes in the presence of nucleic acids
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Interaction of cyanine dyes with nucleic acids. XXV. Influence of affinity-modifying groups in the structure of benzothiazol-4-[2,6-dimethylpyridinium] dyes on the spectral properties of the dyes in the presence of nucleic acids

机译:花青染料与核酸的相互作用。二十五。核酸存在下苯并噻唑-4- [2,6-二甲基吡啶]染料结构中亲和改性基团对染料光谱特性的影响

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Novel monomethine pyridinium cyanine dyes of similar structure and containing 'affinity-modifying groups of different chemical nature were studied by spectral-luminescent methods as possible fluorescent probes for the nucleic acids detection. It was shown that the nature of the functional groups in the dye linker influences the fluorescent properties of the dye-nucleic acids complexes. Incorporation of a hydroxyl group into the linker structure leads to a significant increase in the fluorescence intensity of the dye - double-stranded DNA complexes relative to the parent dye Cyan 40. (C) 2001 Elsevier Science B.V. All rights reserved. [References: 13]
机译:通过光谱发光方法研究了结构相似,含有不同化学性质的'亲和修饰基团的新型单次甲基吡啶鎓花青染料,作为可能的核酸检测探针。结果表明,染料接头中官能团的性质影响染料-核酸复合物的荧光性质。相对于母体染料Cyan 40,将羟基结合到接头结构中导致染料-双链DNA复合物的荧光强度显着增加。(C)2001 Elsevier Science B.V.保留所有权利。 [参考:13]

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