首页> 美国政府科技报告 >Monoclonal Antibodies of Three Different Immunoglobulin G Isotypes Produced byImmunization with a Synthetic Peptide or Native Protein Protect Mice against Challenge with Plasmodium Yoelii Sporozoites. (Reannouncement with New Availability Information)

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Monoclonal Antibodies of Three Different Immunoglobulin G Isotypes Produced byImmunization with a Synthetic Peptide or Native Protein Protect Mice against Challenge with Plasmodium Yoelii Sporozoites. (Reannouncement with New Availability Information)

机译：通过用合成肽或天然蛋白质免疫小鼠产生的三种不同免疫球蛋白G同种型的单克隆抗体保护小鼠免受恶性疟原虫子孢子的攻击。（重新公布新的可用性信息）

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Passive transfer of monoclonal antibodies (MAbs) against malaria circumsporozoite(CS) proteins protects animals against malaria. Active immunization with synthetic or recombinant peptides induces a level of polyclonal antibodies to sporozoites comparable to those found after passive immunization but does not provide comparable protection. In the plasmodium yoelii system, synthetic or recombinant peptide-induced antibodies have never been shown to protect. The current studies were designed to determine whether immunogen structure (native protein versus synthetic peptide) or immunoglobulin G (IgG) subclass of antibodies was responsible for the absolute differences between protective, passively transferred MAbs and nonprotective, actively induced polyclonal antibodies. In this study we produced two MAbs, QGP.S1 (IgG1) and QGP.S2 (IgG2b), by immunization with a synthetic peptide based on the P. yoelii CS major repeat, (QGPGAP)49 conjugated to keyhole limpet hemocyanin. These MAbs were compared to NYS1 (IgG3), an anti-CS protein MAb previously produced by immunization with irradiated P. yoelii sporozoites, which recognizes (QGPGAP)2. QGP.S1 and QGP.S2 passively transferred protection. However, when compared with NYS1, there was a hierarchy of protection, NYS1 > QGP.S1 > QGP.S2. There was no correlation between antibody level at challenge as determined by immunofluorescent antibody test against sporozoites or enzyme-linked immunosorbent assay against (QGPGAP)2 or apparent antibody avidity for (QGPGAP)2 by sodium thiocyanate elution assay. The data demonstrate that a synthetic peptide can induce protective antibodies and that a specific antibody subclass is not required for protection.

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Mucide, A. K.; Bower, J. H.; Hoffman, S. L.; Sedegah, M.; Lees, A.;
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年度 1993
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总页数 6
原文格式 PDF
正文语种 eng
中图分类工业技术;
关键词
Immunization; Peptides; Sporozoites; Antibodies; Immunoglobulins; Proteins; Malaria; Reprints; Immunogens; Immunoassay;

机译：免疫接种;肽;孢子体;抗体;免疫球蛋白;蛋白质;疟疾;重印;免疫原;免疫;

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1. Monoclonal antibodies of three different immunoglobulin G isotypes produced by immunization with a synthetic peptide or native protein protect mice against challenge with Plasmodium yoelii sporozoites. [J] . M Ak, J H Bower, S L Hoffman, Infection and immunity . 1993,第6期

机译：通过合成肽或天然蛋白免疫产生的三种不同免疫球蛋白G同种型的单克隆抗体可保护小鼠免受约氏疟原虫子孢子的攻击。
2. Antimalarial antibodies of the immunoglobulin G2a isotype modulate parasitemias in mice infected with Plasmodium yoelii. [J] . W I White, C B Evans, D W Taylor Infection and immunity . 1991,第10期

机译：免疫球蛋白G2a同种型的抗疟抗体可调节被约氏疟原虫感染的小鼠体内的寄生虫病。
3. Immunization with a recombinant C-terminal fragment of Plasmodium yoelii merozoite surface protein 1 protects mice against homologous but not heterologous P. yoelii sporozoite challenge. [J] . L Rénia, I T Ling, M Marussig, Infection and immunity . 1997,第11期

机译：约氏疟原虫裂殖子表面蛋白1的重组C端片段的免疫保护小鼠免受同源但不异源的约氏疟原虫子孢子攻击。
4. CHARACTERIZATION OF THE EPITOPE FOR ANTI HUMAN RESPIRATORY SYNCYTIAL VIRUS F PROTEIN MONOCLONAL ANTIBODY 101F USING SYNTHETIC PEPTIDES AND RECOMBINANT F PROTEIN MUTANTS [C] . Marian Kruszynski, Sheng Jiun Wu, Albert Schmidt the European Peptide Symposium . 2007

机译：用合成肽和重组F蛋白突变体表征抗人呼吸道合胞病毒F蛋白单克隆抗体101f的表位
5. Monoclonal antibodies of three different immunoglobulin G isotypes produced by immunization with a synthetic peptide or native protein protect mice against challenge with Plasmodium yoelii sporozoites. [O] . M Ak, J H Bower, S L Hoffman, 1993

机译：通过合成肽或天然蛋白免疫产生的三种不同免疫球蛋白G同种型的单克隆抗体可保护小鼠免受约氏疟原虫子孢子的攻击。
6. Antibodies against Ribosomal Phosphoprotein P0 of Plasmodium falciparum Protect Mice against Challenge with Plasmodium yoelii [O] . Chatterjee, Sanchita, Singh, Subhash, Sohoni, Rashmi, 2000

机译：抗恶性疟原虫核糖体磷酸蛋白P0的抗体可保护小鼠免受约氏疟原虫的攻击
7. T Cell Clone Directed at the Circumsporozoite Protein which Protects Mice againstBoth Plasmodium yoelii and Plasmodium berghei. (Reannouncement with New Availability Information) [R] . Weiss, W. R., Berzofsky, J. A., Houghten, R. A., 1992

机译：T细胞克隆针对环子孢子蛋白，保护小鼠免受约氏疟原虫和伯氏疟原虫的侵害。（重新公布新的可用性信息）

Monoclonal Antibodies of Three Different Immunoglobulin G Isotypes Produced byImmunization with a Synthetic Peptide or Native Protein Protect Mice against Challenge with Plasmodium Yoelii Sporozoites. (Reannouncement with New Availability Information)

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