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首页> 美国政府科技报告 >Recognition of Double Helical DNA by Alternate Strand Triple Helix Formation
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Recognition of Double Helical DNA by Alternate Strand Triple Helix Formation

机译：双链三螺旋形成对双螺旋DNa的识别

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摘要

The triplet specificities and required strand orientations of two classes of DNAtriple helices can be combined to target double helical sequences containing all four base pairs by alternate strand triple helix formation. This allows for the use of oligonucleotides containing only natural 3'-5' phosphodiester linkages to simultaneously bind both strands of double helical DNA in the major groove. The stabilities and structures of these alternate strand triple helices depend on whether the binding site sequence is 5'-(purine)m(pyrimidine)n-3' or 5'(pyrimidine)m(purine)n-3'. The sequence type 5'-(purine)m(pyrimidine)n-3' was targeted with an oligonucleotide consisting of a pyrimidine domain and a purine domain linked by a 3'-5' phosphodiester. To bind the duplex sequence type 5'(pyrimidine)m(purine)n-3', the third strand requires at least two nucleotides linking binding domains at the site of crossover in the major groove. Since both of these sequence types have been successfully targeted, a new class of oligonucleotides capable of binding a variety of duplex sequences by multiple crossovers in the major groove may now be possible.

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