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Photochlorophyllide Reductase. 1. Determination of the Regiochemistry and the Stereochemistry of the Reduction of Protochlorophyllide to Chlorophyllide.

机译:光氯叶绿素还原酶。 1.原叶绿素还原为叶绿素的区域化学和立体化学的测定。

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It has been estimated that over 1 billion tons of chlorophyll are synthesized in the biosphere each year. Although the chlorophyll biosynthetic pathway has been the subject of intense investigation over the past 50 years, much remains to be discovered. One promising area of investigation is the remarkable color change, from yellow to green, that occurs when dark-grown plants are exposed to light. This 'greening reaction' involves the reduction of protochlorophyllide to chlorophyllide and is a key regulatory step both in the biosynthesis of chlorophyll and in the development of the chloroplast. This reaction is catalyzed by the enzyme protochlorophyllide reductase and is of interest because it is one of only two known light-requiring enzymatic reactions. The enzyme has been purified from oats, barley, wheat, and squash. It is an unstable membrane-bound protein of 37000 D molecular weight. The quantum yield for protochlorophyllide reduction in barley seedlings is 0.4 and the action spectrum for the reaction in corn seedlings follows the absorption spectrum of protochlorophyllide. Reprints. (jes)

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