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首页> 美国政府科技报告 >Effect of Colchicine on Collagen Synthesis by Liver Fibroblasts in Murine Schistosomiasis. (Reannouncement with New Availability Information).
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Effect of Colchicine on Collagen Synthesis by Liver Fibroblasts in Murine Schistosomiasis. (Reannouncement with New Availability Information).

机译：秋水仙碱对小鼠血吸虫病肝成纤维细胞胶原合成的影响。 （重新公布新的可用性信息）。

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Colchicine, an antimicrotubular agent, was shown to block the transcellular movement of certain structural macromolecules such as collagen. In the present study, the effect of colchicine on collagen synthesis and secretion by monolayer cultures of fibroblasts from livers of mice infected with Schistosoma mansoni was investigated. The effect of colchicine on proliferation of these fibroblast was studied as well. Collagen and non-collagen protein synthesis was measured by incubating cultures with proline and measuring the incorporation of radioactivity into these protein fractions in both culture media and cell layers. Proliferation was measured by thymidine uptake. The isolated fibroblasts actively formed collagen and secreted most of it into the culture medium; 10-20% of the collagenase-sensitive radio-active protein remained in the cell layer. The addition of colchicine to culture medium led to selective inhibition of collagen formation with negligible effects on non-collagen protein synthesis.

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