首页> 美国政府科技报告 >Changes in Canine Neutrophil Function(s) Following Cellular Isolation by Percoll Gradient Centrifugation or Isotonic Lysis

【24h】

Changes in Canine Neutrophil Function(s) Following Cellular Isolation by Percoll Gradient Centrifugation or Isotonic Lysis

机译：percoll梯度离心或等渗裂解细胞分离后犬中性粒细胞功能的变化

获取原文

页面导航

摘要
著录项
相似文献
相关主题

摘要

Analysis of cellular effector function(s) often requires their isolation from other cellular types. Cell separatory techniques could mask, or select out, clinically important functional lesions. We examined differences in canine peripheral blood neutrophil functions, i.e. migration and Hydrogen Peroxide production, following two commonly used cell separation techniques: isotonic lysis or density gradient (Percoll) centrifugation. Separation methodology was observed to have a significant impact on both metabolic and mobility functions. In comparison to isotonic lysis, Percoll separation caused near 100% increases in random migration, near 40% decreases in chemotaxis and 70% increases in H202 production. Reprints. (AW)

著录项

作者
Gruber, D. F.; D'Alesandro, M. M.;
展开▼
作者单位

展开▼
年度 1988
页码 p.1-17
总页数 17
原文格式 PDF
正文语种 eng
中图分类工业技术;
关键词
Cells(Biology); Centrifuge separation; Lysis; Phagocytes; Chemotaxis; Functions; Gradients; Hydrogen peroxide; Isolation; Lesions; Metabolism; Methodology; Migration; Mobility; Production; Reprints; Separation; Dogs;

机译：细胞（生物学）;离心分离;裂解;吞噬细胞;趋化性;功能;梯度;过氧化氢;分离;病变;代谢;方法学;迁移;迁移;生产;重印;分离;狗;

相似文献

外文文献
中文文献
专利

1. Discontinuous Percoll gradient centrifugation combined with immunomagnetic separation obviates the need for erythrocyte lysis and yields isolated eosinophils with minimal granule abnormalities. [J] . Malm Erjefalt M, Stevens TR, Persson CG, Journal of Immunological Methods . 2004,第1a2期

机译：不连续的Percoll梯度离心与免疫磁分离相结合，消除了对红细胞溶解的需要，并产生了具有最小颗粒异常的分离的嗜酸性粒细胞。
2. Apoptosis-like change, ROS, and DNA status in cryopreserved canine sperm recovered by glass wool filtration and Percoll gradient centrifugation techniques [J] . Kim Sue-Hee, Yu Do-Hyeon, Kim Yong-Jun Animal Reproduction Science . 2010,第1a2期

机译：通过玻璃棉过滤和Percoll梯度离心技术回收的冻存犬精子中的凋亡样变化，ROS和DNA状态
3. Separation of canine epididymal spermatozoa by Percoll gradient centrifugation [J] . Hishinuma M, Sekine J Theriogenology . 2004,第2a3期

机译：Percoll梯度离心分离犬附睾精子
4. Effect of Sexing Process Using Percoll Density Gradient Centrifugation and Frozen on Motility And Damage to Spermatozoa Membrane of Filial Ongole [C] . Trinil Susilawati, Enike Dwi Kusumawati, Nurul Isnaini, International Conference in One Health . 2018

机译：使用栓塞密度梯度离心和冷冻对孝戈尔的精子膜对运动和损伤的影响
5. The effect of in vivo administration of canine cytokines on neutrophil production and function. [D] . Farese, Ann Marie. 1993

机译：体内施用犬细胞因子对中性粒细胞产生和功能的影响。
6. Isolation of Spinach Leaf Peroxisomes in 0.25 Molar Sucrose Solution by Percoll Density Gradient Centrifugation [O] . Zheng Liang, Charles Yu, Anthony H. C. Huang 1982

机译：Percoll密度梯度离心分离0.25摩尔蔗糖溶液中的菠菜叶片过氧化物酶体
7. Evaluation of three substitutes for Percoll in sperm isolation by density gradient centrifugation [O] . O. E. Claassens, R. Menkveld, K. L. Harrison 1998

机译：用密度梯度离心评估精子隔离中的三个替代品的替代品

Changes in Canine Neutrophil Function(s) Following Cellular Isolation by Percoll Gradient Centrifugation or Isotonic Lysis

摘要

著录项

相似文献

相关主题

期刊订阅