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首页> 美国政府科技报告 >Use of DNA Probes and HEp-2 Cell Adherence Assay to Detect Diarrheagenic Escherichia coli. (Reannouncement with New Availability Information).
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Use of DNA Probes and HEp-2 Cell Adherence Assay to Detect Diarrheagenic Escherichia coli. (Reannouncement with New Availability Information).

机译：使用DNa探针和HEp-2细胞粘附试验检测腹泻性大肠埃希菌。 （重新公布新的可用性信息）。

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Four major categories of Escherichia coli are recognized as causes of diarrheal disease, including enterotoxigenic E. coli (ETEC), enteropathogenic E. coli (EPEC), enteroinvasive E. coli (EIEC), and enterohemorrhagic E. coli (EHEC); each category comprises strains of distinct O:H serotypes. The development of DNA probes has greatly facilitated the identification of each category of diarrheagenic E. coli and has allowed the categories to be distinguished from one another and from the normal intestinal flora. Another helpful technique in detecting certain E. coli associated with diarrhea is the HEp-2 cell assay, wherein bacteria are incubated for several hours on monolayers of HEp-2 cells, after which the cells are stained and examined for bacterial adherence. Three patterns of adherence of E. coli to HEp-2 cells can be discerned: localized, true diffuse, and aggregate (previously called autoagglutinating). EPEC, the first category of diarrheagenic E. coli to be described, have a particularly rich and controversial history. In the early 1970s, some investigators expressed doubt about the inherent pathogenicity of EPEC, because they did not elaborate on recognized enterotoxins or manifest Shigella-like invasiveness ( as found in ETEC and EIEC, respectively); the value of serogrouping as a diagnostic tool for EPEC was also questioned. Volunteer studies incontestably demonstrated the capacity of EPEC to cause diarrhea and rekindled interest in EPEC's pathogenesis.

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