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Serodiagnosis of Typhoid Fever in Pediatric Patients by Anti-LPS (Lipopolysaccharide) ELISA (Enzyme Linked Immunosorbent Assay)

机译：抗Lps（脂多糖）ELIsa（酶联免疫吸附试验）对儿科患者伤寒的血清学诊断

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Enzyme-linked immunosorbent assay (ELISA) and immunoblot analysis showed that up to 50% of the anti-typhoid antibody in sera from blood culture positive pediatric typhoid fever patients is directed against lipopolysaccharide (LPS) antigen. Anti-Salmonella typhi LPS ELISA was therefore compared to Widal agglutination for serodiagnosis of typhoid fever in pediatric patients. Sera from 38 pediatric control individuals were ELISA negative for anti-S. typhi LPS IgG; all but 2 of these specimens were negative for anti-S. typhi LPS IgM. Pediatric patients hospitalized with signs and symptoms of typhoid fever were separated into 4 groups and tested by ELISA with the following results: 46 patients negative by both culture and widal agglutination test, 48% positive for anti-S. typhi LPS IgG and 35 % for anti-S. typhi LPS IgM; 22 negative by culture but with positive Widal titres, 82% and 68% positive respectively; 28 culture positive for S typhi, 93% and 82% respectively; and 12 culture positive for Salmonella other than S. typhi, 92% and 92% respectively. These data suggest that anti-S. typhi LPS ELISA is a suitable assay for diagnosis of typhoid fever in children. Reprints. (aw)

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Sippel, J. E.; Hanafy, H. M.; Diab, A. S.; Prato, C.; Arroyo, R.;
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年度 1986
页码 p.1-7
总页数 7
原文格式 PDF
正文语种 eng
中图分类工业技术;
关键词
Diagnosis(Medicine); Immunoassay; Serodiagnosis; Typhoid; Agglutination; Children; Enzymes; Fevers; Patients; Pediatrics; Reprints; Salmonella; Signs and symptoms; Test and evaluation; Lipopolysaccharides; Immunoglobulins; Antigen antibody reactions;

机译：诊断（医学）;免疫测定;血清学诊断;伤寒;凝集;儿童;酶;发热;患者;儿科;重印;沙门氏菌;体征和症状;测试和评估;脂多糖;免疫球蛋白;抗原抗体反应;

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1. Indirect immunoglobulin G (IgG) and IgM enzyme-linked immunosorbent assays (ELISAs) and IgM capture ELISA for detection of antibodies to lipopolysaccharide in adult typhoid fever patients in Pakistan. [J] . J Sippel, N Bukhtiari, M B Awan, Journal of Clinical Microbiology . 1989,第6期

机译：间接免疫球蛋白G（IgG）和IgM酶联免疫吸附测定（ELISA）和IgM捕获ELISA用于检测巴基斯坦成年伤寒患者脂多糖的抗体。
2. Serodiagnosis of typhoid fever by enzyme-linked immunosorbent assay determination of anti-Salmonella typhi lipopolysaccharide antibodies. [J] . S Nardiello, T Pizzella, M Russo, Journal of Clinical Microbiology . 1984,第4期

机译：通过酶联免疫吸附测定抗伤寒沙门氏菌脂多糖抗体的血清诊断伤寒。
3. Differential Sensitivities of Severe Acute Respiratory Syndrome (SARS) Coronavirus Spike Polypeptide Enzyme-Linked Immunosorbent Assay (ELISA) and SARS Coronavirus Nucleocapsid Protein ELISA for Serodiagnosis of SARS Coronavirus Pneumonia [J] . Patrick C. Y. Woo, Susanna K. P. Lau, Beatrice H. L. Wong, Journal of Clinical Microbiology . 2005,第7期

机译：严重急性呼吸系统综合症（SARS）冠状病毒穗状多肽酶联免疫吸附测定（ELISA）和SARS冠状病毒核衣夹蛋白ELISA对SARS冠状病毒肺炎的血清学诊断的敏感性差异
4. A method to extract spots from the image of the ELISA (enzyme-linked immunosorbent assay) spot assay [C] . Chih-Yang Lin, Yu-Tai Ching, Wu-Hsieh, Engineering in Medicine and Biology Society, 2001. Proceedings of the 23rd Annual International Conference of the IEEE . 2001

机译：一种从ELISA图像中提取斑点的方法（酶联免疫吸附测定）
5. Development of a monoclonal antibody-based antigen detection enzyme-linked immunosorbent assay (ELISA) for the diagnosis of human toxoplasmosis. [D] . Grushka, Daniel. 2001

机译：基于单克隆抗体的抗原检测酶联免疫吸附测定（ELISA）的开发，用于诊断人类弓形虫病。
6. Indirect immunoglobulin G (IgG) and IgM enzyme-linked immunosorbent assays (ELISAs) and IgM capture ELISA for detection of antibodies to lipopolysaccharide in adult typhoid fever patients in Pakistan. [O] . J Sippel, N Bukhtiari, M B Awan, 1989

机译：间接免疫球蛋白G（IgG）和IgM酶联免疫吸附测定（ELISAs）以及IgM捕获ELISA用于检测巴基斯坦成年伤寒患者脂多糖的抗体。
7. Serodiagnosis of typhoid fever by enzyme-linked immunosorbent assay determination of anti-Salmonella typhi lipopolysaccharide antibodies [O] . S Nardiello, T Pizzella, M Russo, 1984

机译：酶联免疫吸附试验测定抗沙门氏菌抗原脂多糖抗体的血清诊断
8. Indirect Immunoglobulin G (IgG) and IgM Enzyme-Linked Immunosorbent Assays (ELISAs) and IgM Capture ELISA for Detection of Antibodies to Lipopolysaccharide in Adult Typhoid Fever Patients in Pakistan. [R] . Sippel, J., Bukhtiari, N., Awan, M. B., 1989

机译：间接免疫球蛋白G（IgG）和Igm酶联免疫吸附试验（ELIsas）和Igm捕获ELIsa检测巴基斯坦成年伤寒患者脂多糖抗体。

Serodiagnosis of Typhoid Fever in Pediatric Patients by Anti-LPS (Lipopolysaccharide) ELISA (Enzyme Linked Immunosorbent Assay)

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