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首页> 美国政府科技报告 >Distinct Patterns of Transmembrane Calcium Flux and Intracellular Calcium Mobilization after Differentiation Antigen Cluster 2 (E Rosette Receptor) or 3 (T3) Stimulation of Human Lymphocytes
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Distinct Patterns of Transmembrane Calcium Flux and Intracellular Calcium Mobilization after Differentiation Antigen Cluster 2 (E Rosette Receptor) or 3 (T3) Stimulation of Human Lymphocytes

机译：分化抗原簇2（E玫瑰花结受体）或3（T3）刺激人淋巴细胞后跨膜钙通量和细胞内钙动员的不同模式

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We evaluated CD2 (E rosette) and CD3 (T3)-triggered activation of resting lymphocytes by measuring the interacellular free calcium concentration (Ca(2+))i of individual cells. The (Ca(2+)) of indo-1-loaded cells was measured by flow cytometry and responses were correlated with cell surface phenotype. Stimulation with anti-CD3 antibody caused an increase in (Ca(2+))i in > 90% of CD3+ cells within 1 min, and furthermore, the response was restricted to cells bearing the CD3 marker. In contrast, stimulation of cells with anti-CD2 antibodies produced a biphasic response pattern with an early component in CD3(-) cells and a late component in CD3(+) cells. Thus, the CD2 response does not require cell surface expression of CD3. In addition, stimulation of a single CD2 epitope was sufficient for activation of CD3(-) cells, whereas stimulation of two CD2 epitope was required for activation of CD3(+) cells. Both the CD2 and CD3 responses were diminished in magnitude and duration by EGTA. However, approx. 50% of T cells still had a brief response in the presence of EGTA, indicating that the increased (Ca(2+))i results in part from intracellular calcium mobilization, and furthermore demonstrates that extracellular calcium is required for a full and sustained response. Our results support the concept that CD2 represents the trigger for a distinct pathway of activation both for T cells that express the CD3 molecular complex and for large granular lymphocytes that do not.

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