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首页> 美国政府科技报告 >Enhanced Direct Electron Transport with Glucose Oxidase Immobilized on (Aminophenyl)Boronic Acid Modified Glassy Carbon Electrode
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Enhanced Direct Electron Transport with Glucose Oxidase Immobilized on (Aminophenyl)Boronic Acid Modified Glassy Carbon Electrode

机译：用（氨基苯基）硼酸修饰玻碳电极固定化葡萄糖氧化酶增强直接电子输运

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摘要

The immobilization of enzymes in a manner that promotes direct electron transfer between the enzyme cofactor and an electrode surface is of considerable interest for the development of biosensors having high substrate specificity. Direct electron transfer between immobilized glucose oxidase (GO) and (aminophenyl) boronic acid modified glassy carbon (GC) electrodes was observed in the present study. Surface carboxylic acid groups were generated on glassy carbon rods by chemical oxidation. (3-Aminophenyl)boronic acid (APBA) was coupled to the carboxylic acid functions after activation with water-soluble carbodiimide to give GC-APBA electrodes. Interactions with GO furnished GC-APBA-GO electrodes with enzyme immobilized presumably by complex formation of the sugar portion of the enzyme with the boronic acid groupings on the GC surface. This is the first report of the immobilization of an enzyme to an electrode surface through boronate groups. Direct electron transfer between the enyme cofactor and the electrode was demonstrated by cyclic and differential pulse voltammetry. No significant electron transfer was observed with GO in solution either alone or with added APBA. Possible explanations are examined for the direct electron transfer.

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