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'In Vitro' Function of Granulocytes Isolated from Blood of Normal Volunteers Using Continuous-Flow Centrifugation in the IBM-Aminco Celltrifuge and Adhesion Filtration Leukapheresis Using Nylon Fiber.

机译：使用尼龙纤维在IBm-aminco细胞离心机和粘附过滤白细胞分离术中使用连续流动离心从正常志愿者血液中分离的粒细胞的“体外”功能。

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Granulocytes were harvested from each of five healthy male volunteers once by continuous flow centrifugation with the IBM-Aminco Celltrifuge, and once by adhesion filtration leukapheresis with nylon fiber. Granulocyte recovery and purity were significantly better with the filtration leukapheresis system than with the continuous-flow centrifugation. Measurements of trypan blue dye exclusion and muramidase activity were similar to those in control granulocytes regardless of the method of isolation. Granulocyte-stimulated oxygen consumption was diminished in granulocytes prepared by the adhesion filtration method, but normal in those prepared by continuous flow centrifugation with the IBM-Aminco Celltrifuge.

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Wade, P. H.; Skrabut, E. M.; Vinciguerra, L.; Valeri, C. R.;
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年度 1975
页码 1-7
总页数 7
原文格式 PDF
正文语种 eng
中图分类工业技术;
关键词
Granulocytes; Centrifuge separation; Continuous processing; Filtration; Adhesion; Nylon; Synthetic fibers; Oxygen consumption; Reprints; Leukapheresis;

机译：粒细胞;离心分离;连续处理;过滤;粘附;尼龙;合成纤维;耗氧量;重印;白细胞分离;

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专利

1. A comparative trial of granulocyte-colony-stimulating factor and dexamethasone, separately and in combination, for the mobilization of neutrophils in the peripheral blood of normal volunteers. [J] . Liles WC, Huang JE, Llewellyn C, Transfusion: The Journal of the American Association of Blood Banks . 1997,第2期

机译：粒细胞集落刺激因子和地塞米松分别或联合用于正常志愿者外周血中性粒细胞动员的比较试验。
2. The Effects of Recombinant Human Granulocyte-Macrophage Colony Stimulating Factor on in Vitro Cord Blood Granulocyte Function [J] . Robert W Frenck, E Stephen Buescher, Saroj Vadhan-Raj Pediatric Research . 1989,第1期

机译：重组人粒细胞-巨噬细胞集落刺激因子对体外脐血粒细胞功能的影响
3. Glucocorticosteroids and in vitro effects on chemiluminescence of isolated bovine blood granulocytes. [J] . Hoeben D, Burvenich C, Massart-Leen AM European Journal of Pharmacology: An International Journal . 1998,第2a3期

机译：糖皮质激素及其对离体牛血粒细胞化学发光的体外影响。
4. Comparison of the effects of filtration leucapheresis and discontinuous flow centrifugation leucapheresis on granulocyte microbicidal function. [O] . S Martin, A T Ghoneim, E A Robinson, 1983

机译：比较滤过白细胞和不连续流离心白细胞对粒细胞杀微生物功能的影响。
5. In vitro function and post-transfusion survival of granulocytes collected by continuous-flow centrifugation and by filtration leukapheresis [O] . J McCullough, BJ Weiblen, AR Deinard, 1976

机译：通过连续流动离心和过滤白血病收集的粒细胞的体外功能和输出后输送
6. Granulocyte Isolation by Counterflow Centrifugation-Elutriation of Canine Blood Obtained by Continuous Flow Centrifugation Leukapheresis. [R] . Jemionek, J. F., Contreras, T. J., French, J. E., 1978

机译：逆流离心 - 通过连续流动离心白细胞去除术获得的犬血液回流粒细胞分离。

'In Vitro' Function of Granulocytes Isolated from Blood of Normal Volunteers Using Continuous-Flow Centrifugation in the IBM-Aminco Celltrifuge and Adhesion Filtration Leukapheresis Using Nylon Fiber.

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