Changes in Human Erythrocyte Membrane Proteins During Storage

机译：人红细胞膜蛋白在贮藏过程中的变化

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Fresh and 21-day old human blood samples were used to prepare fresh and out-dated stromas. Pooled fresh and outdated samples were solubilized by butanol or pyridine. These two protein fractions (fresh and outdated) produced different patterns on disc electrophoresis and column chromatography. Charge differences between fresh and outdated proteins were shown to be independent of the sialic acid content of the proteins. Conformational changes between fresh and outdated proteins were seen using 1-anilino-8-naphthalene sulfonate (ANS) as a fluorescent probe. Fresh samples showed greater fluorescent enhancement but less total ANS binding, and lower protein-ANS dissociation constants. These changes may cause a reduction in the strength, or integrity, of the erythrocyte membrane and be a limiting factor in the viability of the human red cell during in vitro storage. (Author)

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Moore, G. L.; Cooper, D. A.; Antonoff, R. S.; Robinson, S. L.;
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年度 1970
页码 p.1-20
总页数 20
原文格式 PDF
正文语种 eng
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关键词
Erythrocytes ; Proteins ; Membranes(Biology) ; Storage ; Effectiveness ; Aging(Physiology) ; Hemolysis ; Chromatographic analysis ; Electrophoresis ; pH ; Fluorescence ; Chemical analysis ; Viability ; Sulfonates ; Blood;

机译：红细胞;蛋白质;膜（生物学）;储存;有效性;衰老（生理学）;溶血;色谱分析;电泳; pH;荧光;化学分析;活力;磺酸盐;血液;

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Changes in Human Erythrocyte Membrane Proteins During Storage

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