Development of Rapid Fixation Methods for Neural Tissue

机译：神经组织快速固定方法的研制

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The objective of this program was to develop methods for rapid fixation and preservation of central nervous system tissue. During the first phase of work a perfusion system was developed which would optimally preserve brain tissue with chemical fixatives. Various fixatives were tested and glutaldehyde was found to be the preservative of choice. It penetrated rapidly and maintained structural detail. In order to evaluate the efficiency of the fixative system, histochemical, white light microscopic and isoenzyme evaluations were done. Some reduction in histochemical activity was noted, while white light microscopy indicated good preservation of cellular detail. Isoenzyme evaluations indicated up to 90% loss of activity with chemical fixatives. A system was developed which would freeze brain tissue rapidly for biochemical analyses. Accordingly, a cryogenic probe was designed and constructed which will rapidly remove a plug of brain tissue and freeze it to liquid nitrogen temperature (-190C). This instrument can be operated remotely and will remove and freeze a piece of brain tissue in less than 5 sec. Histochemical and isoenzyme assay of frozen tissue plugs indicate optimal enzyme preservation. (Author)

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作者
Blair, W. H.;
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年度 1967
页码 p.1-62
总页数 62
原文格式 PDF
正文语种 eng
中图分类
关键词
Brain ; Histological techniques ; Central nervous system ; Tissues(Biology) ; Preservation ; Enzymes ; Freezing ; Morphology(Biology) ; Instrumentation;

机译：脑;组织学技术;中枢神经系统;组织（生物学）;保存;酶;冷冻;形态学（生物学）;仪器;

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6. Inner ear tissue preservation by rapid freezing: Improving fixation by high-pressure freezing and hybrid methods [O] . A. Bullen, R.R. Taylor, B. Kachar, -1

机译：通过快速冷冻保存内耳组织：通过高压冷冻和混合方法改善固定
7. Inner ear tissue preservation by rapid freezing: improving fixation by high-pressure freezing and hybrid methods [O] . Bullen A., Taylor R.R., Kachar B., 2014

机译：通过快速冷冻保存内耳组织：通过高压冷冻和混合方法改善固定

Development of Rapid Fixation Methods for Neural Tissue

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