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Preliminary Observations on Testicular Function in Roosters and Mice Exposed to Increased Partial Pressure of Oxygen

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Adult White Leghorn roosters were exposed to 100% oxygen atmospheres at 258 and 380 mm. Hg pressure for 31 and 22 days, respectively. A ground-level control experiment was also performed. Ejaculates were obtained twice weekly by the massage technic and the ejaculate volume, total sperm count, and sperm motility were measured. The results show that confinement to the chamber for 58 days (ground-level control experiment) and exposure to 100% oxygen at 258 mm. Hg pressure for 31 days had essentially no effect on the three variables studied. The ejaculate volume, sperm motility, and total sperm count decreased significantly, however, in roosters exposed to 100% oxygen at 380 mm. Hg; the decrease in the three variables occurred during the first 4 to 7 days. Otherwise, the birds remained apparently healthy throughout the experiments. Histologic examinations were made on the testes after a 30-day recovery period. Size and weight of the testes from the 380 mm. Hg oxygen group were also reduced as compared to controls. Previously, the reproductive capabilities of male and female mice investigated at higher partial pressures were found to be adversely affected. (Author)

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